Yoshino T, Mukuzono H, Aoki H, Takahashi K, Takeuchi T, Kubonishi I, Ohtsuki Y, Motoi M, Akagi T
Department of Pathology, Okayama University Medical School, Japan.
Am J Pathol. 1989 Jun;134(6):1339-46.
A novel monoclonal antibody (MAb), OPD4, reactive with a helper/inducer (H/I) subset of T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with an activated H/I T cell line, namely DL40. The antibody is an IgG1 antibody and it recognizes an antigen with a molecular weight of 200 kd, corresponding to that of leukocyte common antigen. OPD4+/CD4+ T cells provided better help for pokeweed mitogen-stimulated polyclonal IgG production than OPD4-/CD4+ T cells. OPD4 recognized the H/I T cell subset even in paraffin-embedded tissue sections, but did not recognize nonhematopoietic cells, suppressor/cytotoxic T cells, B cells, monocytes in the peripheral blood, or other normal hematopoietic cells as examined by the flow cytometric and immunoperoxidase methods. Besides the lymphoid cells, OPD4 reacted with a number of histiocytes (epithelioid cells) in tissues from sarcoidosis and tuberculosis. For the neoplastic lesions, OPD4 reacted with approximately half of the cases of T cell lymphomas. Consequently, OPD4 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.
通过用活化的辅助/诱导（H/I）T细胞系DL40免疫，已鉴定出一种新型单克隆抗体（MAb）OPD4，它可与福尔马林固定、石蜡包埋组织切片中的T细胞辅助/诱导（H/I）亚群发生反应。该抗体为IgG1抗体，识别分子量为200kd的抗原，与白细胞共同抗原的分子量相对应。与OPD4-/CD4+ T细胞相比，OPD4+/CD4+ T细胞对商陆有丝分裂原刺激的多克隆IgG产生提供了更好的辅助。即使在石蜡包埋组织切片中，OPD4也能识别H/I T细胞亚群，但通过流式细胞术和免疫过氧化物酶法检测，它不识别非造血细胞、抑制/细胞毒性T细胞、B细胞、外周血中的单核细胞或其他正常造血细胞。除淋巴细胞外，OPD4还与结节病和结核病组织中的许多组织细胞（上皮样细胞）发生反应。对于肿瘤性病变，OPD4与大约一半的T细胞淋巴瘤病例发生反应。因此，OPD4可能有助于恶性淋巴瘤和其他相关病变的诊断和研究。
