Elkahoui S, Djébali N, Karkouch I, Ibrahim A Hadj, Kalai L, Bachkovel S, Tabbene O, Limam F
Prikl Biokhim Mikrobiol. 2014 Mar-Apr;50(2):184-8.
This work aims to characterize the bioactive molecules produced by an antagonistic Bacillus sp. strain BCLRB2 isolated from healthy leaves of olive tree against Rhizoctonia solani and Sclerotinia sclerotiorum. The bacterial strain isolated showed a high and persistent antifungal activity against the two pathogens. The free-cell supernatant showed also a high antifungal activity against R. solani and at a lower extent against S. sclerotiorum. The partial purification of the antifungal substances with methanol gradient applied to C18 column binding the Bacillus BCLRB2 culture supernatant showed that the 20% and 60% methanol fractions had a high and specific activity against S. sclerotiorum and R. solani, respectively. The mass spectrometry identification of the compounds in the fraction specifically active against S. sclerotiorum revealed the presence of bacillomycin D C16 as a major lipopeptide. The fraction specifically active against R. solani contained bacillomycin D C15 and 2 unknown lipopeptides. The 80% methanol fraction had a moderate and a broad spectrum activity against the two pathogens and consisted from two iturin D (C13 and C14) as a major lipopeptides.
这项工作旨在表征从橄榄树健康叶片中分离得到的拮抗菌芽孢杆菌属菌株BCLRB2所产生的生物活性分子，该菌株对茄丝核菌和核盘菌具有拮抗作用。分离得到的细菌菌株对这两种病原体表现出高且持久的抗真菌活性。游离细胞上清液对茄丝核菌也表现出高抗真菌活性，对核盘菌的活性较低。将甲醇梯度应用于结合芽孢杆菌BCLRB2培养上清液的C18柱上对抗真菌物质进行部分纯化，结果表明，20%和60%甲醇级分分别对核盘菌和茄丝核菌具有高且特异的活性。对核盘菌具有特异活性的级分中化合物的质谱鉴定显示，主要脂肽为杆菌霉素D C16。对茄丝核菌具有特异活性的级分含有杆菌霉素D C15和2种未知脂肽。80%甲醇级分对这两种病原体具有中等且广谱活性，主要脂肽为两种伊枯草菌素D（C13和C14）。
