Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria, Hammam-Lif, Tunisia.
FEMS Microbiol Lett. 2011 Mar;316(2):108-14. doi: 10.1111/j.1574-6968.2010.02199.x. Epub 2011 Jan 13.
Bacillus subtilis B38, isolated from soil, showed antimicrobial activity against human pathogenic Candida albicans species. Specific PCR primers revealed the presence of the bamC gene, which is involved in the biosynthesis of bacillomycin D. Three anti-Candida compounds designated a(1) , a(2) and a(3) were purified from culture supernatant and identified using matrix-assisted laser desorption/ionization time-of-flight MS as analogues of bacillomycin D-like lipopeptides of 14, 15 and 16 carbon fatty acid long chains, respectively. The compound a(3) displayed the strongest fungicidal activity against pathogenic C. albicans strains. It was even more active than amphotericin B with a lethal concentration of 59.07 vs. 135.26 μM of the antimycotic drug against the pathogenic strain C. albicans sp. 311 isolated from finger nail. Only moderate or weak anti-Candida activity was recorded for a(1) and a(2) compounds. Furthermore, a(3) showed the highest hemolytic activity, reaching 50% hemolysis at 22.14 μM, whereas a(1) and a(2) displayed a limited hemolysis at 68.26 and 37.41 μM, respectively. These findings suggest that the acyl chain length of bacillomycin D-like lipopeptides plays a major role in hemolytic and antifungal activities.
从土壤中分离出的枯草芽孢杆菌 B38 对人体致病性白色念珠菌具有抗菌活性。特异性 PCR 引物显示存在 BamC 基因，该基因参与芽孢杆菌素 D 的生物合成。从培养上清液中纯化出三种抗真菌化合物，分别命名为 a(1)、a(2)和 a(3)，并使用基质辅助激光解吸/电离飞行时间质谱鉴定为 14、15 和 16 个碳长链脂肪酸的芽孢杆菌素 D 类似脂肽。化合物 a(3)对致病性白色念珠菌菌株表现出最强的杀菌活性。它比两性霉素 B 更有效，对从指甲中分离出的致病性白色念珠菌菌株 311 的致死浓度为 59.07 μM，而抗真菌药物为 135.26 μM。化合物 a(1)和 a(2)对白色念珠菌的抗真菌活性仅为中度或弱。此外，a(3)表现出最高的溶血活性，在 22.14 μM 时达到 50%溶血，而 a(1)和 a(2)的溶血活性分别限制在 68.26 和 37.41 μM。这些发现表明芽孢杆菌素 D 类似脂肽的酰链长度在溶血和抗真菌活性中起主要作用。
