Auger E A, Redding K E, Plumb T, Childs L C, Meng S Y, Bennett G N
Department of Biochemistry, Rice University, Houston, Texas 77251.
Mol Microbiol. 1989 May;3(5):609-20. doi: 10.1111/j.1365-2958.1989.tb00208.x.
The induction of several amino acid decarboxylases under anaerobic conditions at low pH has been known for many years, but the mechanism associated with this type of regulation has not been elucidated. To study the regulation of the biodegradative arginine and lysine decarboxylases of Escherichia coli K12, Mudlac fusions to these genes were isolated. Mudlac fusion strains deficient for lysine decarboxylase or arginine decarboxylase were identified using decarboxylase indicator media and analysed for their regulation of beta-galactosidase expression. The position of the Mudlac fusion in lysine decarboxylase-deficient strains has been mapped to the cadA gene at 93.7 minutes, while the Mudlac fusions exhibiting a deficiency in the inducible arginine decarboxylase have been mapped to 93.4 minutes.
多年来人们已经知道，在低pH值的厌氧条件下会诱导几种氨基酸脱羧酶，但与这种调节类型相关的机制尚未阐明。为了研究大肠杆菌K12生物降解性精氨酸和赖氨酸脱羧酶的调节作用，分离了与这些基因的Mudlac融合体。使用脱羧酶指示培养基鉴定了缺乏赖氨酸脱羧酶或精氨酸脱羧酶的Mudlac融合菌株，并分析了它们对β-半乳糖苷酶表达的调节。在赖氨酸脱羧酶缺陷菌株中，Mudlac融合的位置已定位到93.7分钟处的cadA基因，而表现出诱导型精氨酸脱羧酶缺陷的Mudlac融合已定位到93.4分钟处。
