Rameix-Welti Marie-Anne, Le Goffic Ronan, Hervé Pierre-Louis, Sourimant Julien, Rémot Aude, Riffault Sabine, Yu Qin, Galloux Marie, Gault Elyanne, Eléouët Jean-François
1] Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France [2] Physiopathologie et diagnostic des infections microbiennes, EA3647-EPIM, UFR des Sciences de la Santé Simone Veil-UVSQ, 2 avenue de la Source de la Bièvre, 78180 Montigny-Le-Bretonneux, France [3] AP-HP, Hôpital Ambroise Paré, Laboratoire de Microbiologie, Boulogne-Billancourt 92100, France.
Unité de Virologie et Immunologie Moleculaires (UR892), INRA, Jouy-en-Josas F78352, France.
Nat Commun. 2014 Oct 3;5:5104. doi: 10.1038/ncomms6104.
Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.
呼吸道合胞病毒（RSV）是犊牛和幼儿严重下呼吸道疾病的最重要病因，但目前尚无人类疫苗或有效的治疗方法。在此，我们描述了一种重组人RSV反向遗传学系统，其中红色荧光蛋白（mCherry）或萤火虫荧光素酶（Luc）基因被插入到RSV基因组中。mCherry和Luc的表达与感染率相关，从而能够监测RSV在细胞培养中的增殖情况。通过生物发光成像可以观察到编码Luc的病毒在活体小鼠中的复制，经鼻接种后，在感染小鼠的口鼻部和肺部可检测到生物发光。我们认为，这些重组病毒是筛选抗RSV活性化合物的便捷且有价值的工具，并且可作为研究抗病毒疗法在活体小鼠中作用效果的极其灵敏的指标。
