Icahn School of Medicine at Mount Sinai, Department of Microbiology, New York, New York, USA.
J Virol. 2013 Aug;87(15):8272-81. doi: 10.1128/JVI.00969-13. Epub 2013 May 22.
Influenza A virus is a major human pathogen responsible for seasonal epidemics as well as pandemic outbreaks. Due to the continuing burden on human health, the need for new tools to study influenza virus pathogenesis as well as to evaluate new therapeutics is paramount. We report the development of a stable, replication-competent luciferase reporter influenza A virus that can be used for in vivo imaging of viral replication. This imaging is noninvasive and allows for the longitudinal monitoring of infection in living animals. We used this tool to characterize novel monoclonal antibodies that bind the conserved stalk domain of the viral hemagglutinin of H1 and H5 subtypes and protect mice from lethal disease. The use of luciferase reporter influenza viruses allows for new mechanistic studies to expand our knowledge of virus-induced disease and provides a new quantitative method to evaluate future antiviral therapies.
甲型流感病毒是一种主要的人类病原体，可导致季节性流行和大流行爆发。由于持续对人类健康造成负担，因此需要新的工具来研究流感病毒发病机制以及评估新的疗法。我们报告了一种稳定的、具有复制能力的荧光素酶报告流感病毒的开发，该病毒可用于病毒复制的体内成像。这种成像方法是非侵入性的，可以在活体内对感染进行纵向监测。我们使用该工具来表征新型单克隆抗体，这些抗体结合了 H1 和 H5 亚型病毒血凝素的保守茎部结构域，并保护小鼠免受致命疾病的侵害。荧光素酶报告流感病毒的使用允许进行新的机制研究，以扩展我们对病毒引起的疾病的认识，并提供了一种新的定量方法来评估未来的抗病毒疗法。
