Eléouët Jean-François, Galloux Marie, Rameix-Welti Marie-Anne
Unité de Virologie et Immunologie Moléculaires (VIM) UMR0892, INRAE-UVSQ-Université Paris-Saclay, Jouy-en-Josas, France.
Unit M3P Institut Pasteur, Université Paris-Saclay, Université de Versailles St. Quentin, Université Paris Cité, UMR 1173 (2I), INSERM; Assistance Publique des Hôpitaux de Paris, Centre National de Référence Virus des Infections Respiratoire (CNR VIR), Paris, France.
Methods Mol Biol. 2025;2948:281-288. doi: 10.1007/978-1-0716-4666-3_18.
Modeling human respiratory syncytial virus (RSV) infection in vivo is an essential step in the search for novel vaccines, antiviral therapies, or preventive measures against RSV disease. The most commonly used experimental models of RSV infection are rodent models, in particular, inbred BALB/c mice and cotton rats (Bem et al., Am J Physiol Lung Cell Mol Physiol 301(2): L148-L156, 2011; Taylor, Vaccine 35(3): 469-480, 2017; Altamirano-Lagos, Front Microbiol 10: 873, 2019). However, depending on the RSV strain and viral titer, infection of mice with RSV can result in few or no symptoms, with viral load being estimated by RT-qPCR on lung tissue. By allowing the monitoring of the spread and intensity of the infection in the same animal over time, the use of recombinant RSV expressing the firefly luciferase represents a major advance in the study of RSV replication in the murine model.
在体内模拟人类呼吸道合胞病毒（RSV）感染是寻找新型疫苗、抗病毒疗法或预防RSV疾病措施的关键一步。最常用的RSV感染实验模型是啮齿动物模型，特别是近交系BALB/c小鼠和棉鼠（Bem等人，《美国生理学杂志：肺细胞与分子生理学》301(2): L148-L156，2011年；Taylor，《疫苗》35(3): 469-480，2017年；Altamirano-Lagos，《微生物学前沿》10: 873，2019年）。然而，根据RSV毒株和病毒滴度的不同，用RSV感染小鼠可能几乎不产生症状或完全不产生症状，需通过对肺组织进行逆转录定量聚合酶链反应（RT-qPCR）来估计病毒载量。通过监测同一动物体内感染随时间的传播和强度，使用表达萤火虫荧光素酶的重组RSV代表了小鼠模型中RSV复制研究的一项重大进展。
