在乳铁蛋白固定化钛基底上生长可增强MG-63细胞的成骨细胞活性。
Osteoblast activity of MG-63 cells is enhanced by growth on a lactoferrin-immobilized titanium substrate.
作者信息
Kim Sung Eun, Yun Young-Pil, Lee Jae Yong, Park Kyeongsoon, Suh Dong Hun
机构信息
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.
Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu 136-701, Republic of Korea.
出版信息
Colloids Surf B Biointerfaces. 2014 Nov 1;123:191-8. doi: 10.1016/j.colsurfb.2014.09.014. Epub 2014 Sep 23.
The aim of this study was to develop a lactoferrin (LF)-immobilized titanium (Ti) substrate to enhance the osteoblast activity of MG-63 cells. Ti substrates were first modified through heparin-dopamine (Hep-DOPA) anchorage. Then, LF was immobilized on the Hep-Ti substrates via electrostatic interactions. Hep-Ti substrates, with or without LF, were evaluated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Sustained release of LF on the Ti substrates was observed over a 28-day period. In vitro studies of osteoblast activity showed increased alkaline phosphatase activity and calcium deposition by MG-63 cells cultured on LF-immobilized Ti substrates as compared to those cultured on pristine Ti substrates, indicating that LF-immobilized Ti substrates were effective at enhancing osteoblast activity.
本研究的目的是开发一种固定化乳铁蛋白（LF）的钛（Ti）基底，以增强MG-63细胞的成骨细胞活性。首先通过肝素-多巴胺（Hep-DOPA）锚定对Ti基底进行修饰。然后，通过静电相互作用将LF固定在Hep-Ti基底上。通过扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和接触角测量对有无LF的Hep-Ti基底进行评估。在28天的时间里观察到LF在Ti基底上的持续释放。成骨细胞活性的体外研究表明，与在原始Ti基底上培养的MG-63细胞相比，在固定化LF的Ti基底上培养的MG-63细胞的碱性磷酸酶活性和钙沉积增加，表明固定化LF的Ti基底在增强成骨细胞活性方面是有效的。
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