Noh Sung Hyun, Jo Han-Saem, Choi Somang, Song Hee Gyeong, Kim Hak-Jun, Kim Keung Nyun, Kim Sung Eun, Park Kyeongsoon
Department of Neurosurgery, National Health Insurance Service Ilsan Hospital, #100, Ilsan-ro, Ilsan-donggu, Gyeonggi-do, Goyang-si 10444, Korea.
Department of Systems Biotechnology, Chung-Ang University, Gyeonggi-do, Anseong-si 17546, Korea.
Pharmaceutics. 2020 Dec 26;13(1):30. doi: 10.3390/pharmaceutics13010030.
In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, calcium accumulation, and expression of osteo-differentiation-specific genes, including osteocalcin () and osteopontin (). Both bare MSNs and LF/TA-MSNs exhibited round nano-particle structures. The LF/TA-MSNs demonstrated prolonged LF release for up to 28 days. Treatment of ADSCs with LF (50 μg)/TA-MSNs resulted in markedly higher ALP level and calcium accumulation compared to treatment with LF (10 μg)/TA-MSNs or bare MSNs. Furthermore, LF (50 μg)/TA-MSNs remarkably increased mRNA levels of osteo-differentiation-specific genes, including and , compared to MSNs or LF (10 μg)/TA-MSNs. Together, these data suggest that the ability of LF/TA-MSNs to enhance osteo-differentiation of ADSCs make them a possible nanovehicle for bone healing and bone regeneration in patients with bone defect or disease.
在本研究中，我们制备了负载单宁酸的乳铁蛋白锚定介孔二氧化硅纳米材料（LF/TA-MSNs），并通过检测碱性磷酸酶（ALP）水平、钙沉积以及骨分化特异性基因（包括骨钙素（）和骨桥蛋白（））的表达，评估了这些LF/TA-MSNs对脂肪来源干细胞（ADSCs）体外骨分化能力的影响。裸MSNs和LF/TA-MSNs均呈现出圆形纳米颗粒结构。LF/TA-MSNs显示出长达28天的乳铁蛋白持续释放。与用LF（10μg）/TA-MSNs或裸MSNs处理相比，用LF（50μg）/TA-MSNs处理ADSCs导致显著更高的ALP水平和钙沉积。此外，与MSNs或LF（10μg）/TA-MSNs相比，LF（50μg）/TA-MSNs显著增加了骨分化特异性基因（包括和）的mRNA水平。总之，这些数据表明LF/TA-MSNs增强ADSCs骨分化的能力使其成为骨缺损或疾病患者骨愈合和骨再生的一种可能的纳米载体。
