双相磷酸钙（BCP）固定化多孔聚（d，l-乳酸-共-乙醇酸）微球增强成骨细胞的成骨活性。
Biphasic Calcium Phosphate (BCP)-Immobilized Porous Poly (d,l-Lactic-co-Glycolic Acid) Microspheres Enhance Osteogenic Activities of Osteoblasts.
作者信息
Shim Kyu-Sik, Kim Sung Eun, Yun Young-Pil, Choi Somang, Kim Hak-Jun, Park Kyeongsoon, Song Hae-Ryong
机构信息
Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, Seoul 02841, Korea.
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea.
出版信息
Polymers (Basel). 2017 Jul 21;9(7):297. doi: 10.3390/polym9070297.
The purpose of this study was to evaluate the potential of porous poly (d,l-lactic--glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with l-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin⁻dopamine (Hep-DOPA) to obtain heparinized⁻BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.
本研究的目的是评估固定在双相磷酸钙纳米颗粒（BCP NPs）上的多孔聚（d，l-乳酸-乙醇酸）（PLGA）微球（PMSs）（BCP-IM-PMSs）增强成骨活性的潜力。使用流体装置制备PMSs，并用l-赖氨酸对其表面进行修饰（胺化-PMSs），而用肝素-多巴胺（Hep-DOPA）对BCP NPs进行修饰以获得肝素化-BCP（Hep-BCP） NPs。通过Hep-BCP NPs与胺化-PMSs之间的静电相互作用制备BCP-IM-PMSs。制备的BCP-IM-PMSs显示出相互连接的孔结构。体外研究表明，与在PMSs上培养的细胞相比，在BCP-IM-PMSs上培养的MG-63细胞的碱性磷酸酶活性、钙含量以及骨钙素（OCN）和骨桥蛋白（OPN）的mRNA表达均有所增加。这些数据表明，固定有BCP NP的PMSs具有增强成骨活性的潜力。
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