Wang Gongping, Zeng Guangwei, Wang Caie, Wang Huasheng, Yang Bo, Guan Fangxia, Li Dongpeng, Feng Xiaoshan
Department of Pharmacy, the First Affiliated Hospital of Henan University of Science and Technology, LuoYang 471003 , Henan Province, PR China.
Department of Colorectal Surgery, People Hospital of Zhengzhou, Zhengzhou 450000 , Henan Province, PR China.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2015 Jun;159(2):227-33. doi: 10.5507/bp.2014.045. Epub 2014 Oct 2.
Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling.
Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers.
Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106.
Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.
羊膜来源的间充质干细胞（hAM - dMSCs）是间充质干细胞的一个潜在来源，可用于修复皮肤损伤。使用间充质干细胞修复皮肤损伤需要安全、有效且具有生物相容性的试剂来评估修复效果。由CdSe/ZnS组成的量子点（QDs）是具有宽激发光谱和窄发射光谱的半导体纳米晶体，已被视为一种用于在体外和体内非侵入性标记不同细胞的新型化学和荧光物质。本研究调查了量子点在标记后不同时间对hAM - dMSCs的细胞毒性作用。
使用0.75、1.5和3.0 μL量子点，在第1、2和4天收集标记的人羊膜间充质干细胞，观察形态变化，进行MTT细胞生长测定以及针对间充质干细胞分子标志物的流式细胞术检测。
在荧光显微镜下观察0.75 μg/mL量子点浓度标记的细胞形态，MTT测定显示细胞处于增殖期。流式细胞术检测显示CD29、CD31、CD34、CD44、CD90、CD105和CD106的表达情况。
在一定浓度范围内，量子点标记人羊膜间充质干细胞具有良好的生物相容性。
