Ohyabu Yoshimi, Kaul Zeenia, Yoshioka Tomokazu, Inoue Kazuki, Sakai Shinsuke, Mishima Hajime, Uemura Toshimasa, Kaul Sunil C, Wadhwa Renu
National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Hum Gene Ther. 2009 Mar;20(3):217-24. doi: 10.1089/hum.2008.100.
Progress in stem cell research has prioritized the refinement of cell-labeling techniques for in vitro and in vivo basic and therapeutic studies. Although quantum dots, because of their optical properties, are emerging as favorable nanoparticles for bioimaging, substantial refinements or modifications that would improve their biocompatibility are still required. We report here that internalizing quantum dots (i-QDs) generated by their conjugation with an internalizing antibody against a heat shock protein-70 family stress chaperone, mortalin, offered an efficient, genetically noninvasive, nontoxic, and functionally inert way to label mesenchymal stem cells (MSCs). The i-QD-labeled MSCs underwent normal adipocyte, osteocyte, and chondrocyte differentiation in vitro and in vivo, suggesting the potential application of i-QDs in in vivo diagnostics, regenerative and therapeutic medicine.
干细胞研究的进展已将体外和体内基础研究及治疗研究中细胞标记技术的完善作为优先事项。尽管量子点因其光学特性正成为生物成像领域有利的纳米颗粒，但仍需要进行大量改进或修饰以提高其生物相容性。我们在此报告，通过将量子点与抗热休克蛋白-70家族应激伴侣mortalin的内化抗体偶联而产生的内化量子点（i-QD），为标记间充质干细胞（MSC）提供了一种高效、基因非侵入性、无毒且功能惰性的方法。i-QD标记的MSC在体外和体内均经历了正常的脂肪细胞、骨细胞和软骨细胞分化，这表明i-QD在体内诊断、再生医学和治疗医学中的潜在应用。
