Grzelak I, Olszewski W L, Rowinski W
Surgical Research and Transplantation Laboratory, Experimental and Clinical Medical Research Centre, Polish Academy of Sciences, Warsaw.
Eur Surg Res. 1989;21(2):114-22. doi: 10.1159/000129011.
The in vitro interleukin-1 (IL-1) and interleukin-2 (IL-2) production and IL-2 receptor (IL-2-R) expression by peripheral blood mononuclear (PBM) cells were examined in patients undergoing elective cholecystectomy. The IL-1 production by monocytes stimulated with a lipopolysaccharide (LPS) did not change on day +1 but increased by 57.5% on day +3 (p less than 0.05) after surgery, and was normalized on days +5 and +7. The IL-1 production by PBM cells stimulated by LPS increased by 97.6% on day +1 (p less than 0.05) and 109.5% on day +3 (p less than 0.05) after surgery, and became normalized on days +5 and +7. The IL-2 level in PBM cell supernatants stimulated with phytohemagglutinin (PHA) decreased by 45.2% on day +1 (p less than 0.05), 33.3% on day +3 (p less than 0.05), 32.3% on day +5 (p less than 0.05) and returned to the preoperative values on day +7. The IL-2-R expression on PHA-stimulated lymphocytes increased from 60.4 +/- 4.8% preoperatively to 67.2 +/- 1.9% on day +1 (p less than 0.05) but was again within the preoperative values on days +3, +5 and +7. In order to detect the described changes, stimulation with PHA had to be used. This indicates that simple surgical trauma produces subthreshold activation of blood immune cells. The cells enter the 'response alert' stage which can be measured after stimulation with lectins.(ABSTRACT TRUNCATED AT 250 WORDS)
对接受择期胆囊切除术的患者外周血单个核（PBM）细胞的体外白细胞介素-1（IL-1）和白细胞介素-2（IL-2）生成以及IL-2受体（IL-2-R）表达进行了检测。脂多糖（LPS）刺激单核细胞产生的IL-1在术后第1天未发生变化，但在术后第3天增加了57.5%（p<0.05），并在第5天和第7天恢复正常。LPS刺激PBM细胞产生的IL-1在术后第1天增加了97.6%（p<0.05），在术后第3天增加了109.5%（p<0.05），并在第5天和第7天恢复正常。植物血凝素（PHA）刺激PBM细胞上清液中的IL-2水平在术后第1天下降了45.2%（p<0.05），在术后第3天下降了33.3%（p<0.05），在术后第5天下降了32.3%（p<0.05），并在术后第7天恢复到术前值。PHA刺激的淋巴细胞上IL-2-R的表达从术前的60.4±4.8%增加到术后第1天的67.2±1.9%（p<0.05），但在术后第3天、第5天和第7天又回到了术前值。为了检测上述变化，必须使用PHA刺激。这表明单纯的手术创伤会导致血液免疫细胞产生亚阈值激活。细胞进入“反应警戒”阶段，这可以在用凝集素刺激后进行测量。（摘要截选至250字）
