Yunis J J, Jones C, Madden M T, Lu D, Mayer M G
University of Minnesota Medical School, Minneapolis, Minnesota 55455.
Genomics. 1989 Jul;5(1):84-90. doi: 10.1016/0888-7543(89)90090-6.
Eleven genes were found to be amplified in a patient with acute myelogenous leukemia and a homogeneous staining region 11q23qter. The gene order of such region was determined by using transverse alternating field electrophoresis of normal cell DNA and Southern blots of DNA from somatic cell hybrids, each containing a single human derivative chromosome 11 from six different chromosomal defects. This in turn allowed us to uncover a breakpoint in band 11q23.3 between the CD3 gamma and the ets-1 genes in genomic rearrangements found in acute myelogenous leukemia, acute lymphocytic leukemia, and B-cell diffuse lymphoma. The breakpoint of a constitutional deletion from a patient whose mother and brother have a heritable 11q23.3 fragile site occurs in the same region.
在一名急性髓性白血病患者及具有均一染色区11q23qter的患者中发现11个基因被扩增。通过对正常细胞DNA进行横向交变电场电泳以及对来自体细胞杂种的DNA进行Southern印迹分析来确定该区域的基因顺序，每个体细胞杂种都含有来自六种不同染色体缺陷的单条人类衍生染色体11。这进而使我们能够在急性髓性白血病、急性淋巴细胞白血病和B细胞弥漫性淋巴瘤中发现的基因组重排中，揭示11q23.3带中CD3γ基因和ets-1基因之间的一个断点。一名母亲和兄弟具有遗传性11q23.3脆性位点的患者的一个先天性缺失的断点也出现在同一区域。
