Uguz C, Varisli O, Agca C, Evans T, Agca Y
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA.
Andrologia. 2015 Oct;47(8):910-9. doi: 10.1111/and.12346. Epub 2014 Oct 3.
The objective of this study was to determine the effects of nonylphenol (NP) on viability of ram and boar sperm in vitro. Ram or boar spermatozoa were exposed to 1, 10, 100, 250 and 500 μg NP ml(-1) for 1, 2, 3 or 4 h. Computer-assisted sperm motility analysis (CASA) system was used to evaluate sperm motility characteristics. Flow cytometry was used to determine mitochondrial membrane potential (MMP) and chromatin integrity, while epifluorescent microscopy was used to determine sperm acrosomal status. Exposure of both species spermatozoa to 250 and 500 μg NP ml(-1) was detrimental to progressive motility (P < 0.05), and its adverse effect was significant at lower (100 μg NP ml(-1) ) concentration (P < 0.05). The percentages of ram and boar spermatozoa with high MMP declined drastically after exposures to ≥250 μg ml(-1) NP (P < 0.05). Unlike chromatin integrity, which did not appear to be altered by NP exposure, there were dose-dependent NP effects (P < 0.05) on acrosomal integrity of both species at as low as 1 μg ml(-1) NP for boar spermatozoa and 10 μg ml(-1) NP for ram spermatozoa. These data show adverse effects of NP on ram and boar spermatozoa and thus its potential harmful effects on male reproduction as NP is found in fruits, vegetables, human milk, fish and livestock products.
本研究的目的是确定壬基酚（NP）对体外培养的公羊和公猪精子活力的影响。将公羊或公猪精子暴露于1、10、100、250和500μg NP ml⁻¹中1、2、3或4小时。使用计算机辅助精子活力分析（CASA）系统评估精子活力特征。流式细胞术用于测定线粒体膜电位（MMP）和染色质完整性，而落射荧光显微镜用于测定精子顶体状态。两种物种的精子暴露于250和500μg NP ml⁻¹均对前向运动有不利影响（P < 0.05），且在较低浓度（100μg NP ml⁻¹）时其不良影响显著（P < 0.05）。暴露于≥250μg ml⁻¹ NP后，具有高MMP的公羊和公猪精子百分比急剧下降（P < 0.05）。与染色质完整性不同，NP暴露似乎未改变染色质完整性，对于两种物种的顶体完整性，NP存在剂量依赖性影响（P < 0.05），对于公猪精子，低至1μg ml⁻¹ NP时就有影响，对于公羊精子，低至10μg ml⁻¹ NP时就有影响。这些数据表明NP对公羊和公猪精子有不良影响，因此鉴于在水果、蔬菜、人乳、鱼类和畜产品中发现NP，其可能对雄性生殖有潜在有害影响。
