Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2013;48(8):973-9. doi: 10.1080/10934529.2013.762744.
The objective of this in vitro study was to determine the effect of nonylphenol (NP) as an environmental toxicant on the spermatozoa motility and viability. The dose- and time-dependent effect of nonylphenol (1, 10, 100 and 200 μg/mL) dissolved either in 0.1% dimethyl sulfoxide (DMSO) or 0.1% ethanol (ETOH) on the motility and viability of bovine spermatozoa, as a cell model, during several time periods (0 h, 2 h, 4 h and 6 h) were examined. The motility of spermatozoa was determined by the Sperm Vision(TM) CASA (Computer Assisted Semen Analyzer) system. The results showed decreased spermatozoa motility in all experimental groups with the addition of NP dissolved in 0.1% DMSO and 0.1% ETOH (P < 0.001 and P < 0.05). The lowest spermatozoa motility was found at doses > 100 μg/mL of NP in comparison with the control group. The viability of bovine spermatozoa detected by the MTT cytotoxicity assay was decreased significantly (P < 0.001) in all experimental groups with NP dissolved in 0.1% ETOH. The viability in groups with NP dissolved in 0.1% DMSO was significantly (P < 0.05) decreased at 1 μg/mL of NP and significantly decreased (P < 0.001) at doses > 10 μg/mL of NP. After 6 h of culture the MTT assay proved a negative effect of all NP doses the on cell viability. The obtained data clearly indicate the negative effect of NP as an endocrine disruptor on spermatozoa motility and viability, which should be seriously considered in the case of exposure to NP in animals and humans and as a reason of male reproductive dysfunction.
本体外研究旨在确定环境污染物壬基酚（NP）对精子活力和活力的影响。以牛精子为细胞模型，研究了 NP（1、10、100 和 200μg/ml）在不同剂量和时间（0 h、2 h、4 h 和 6 h）下溶解在 0.1%二甲基亚砜（DMSO）或 0.1%乙醇（ETOH）中的剂量和时间依赖性对精子活力和活力的影响。精子活力通过 Sperm Vision（TM）CASA（计算机辅助精液分析）系统确定。结果表明，加入溶解在 0.1%DMSO 和 0.1%ETOH 中的 NP 的所有实验组的精子活力均下降（P<0.001 和 P<0.05）。与对照组相比，NP 剂量>100μg/ml 时精子活力最低。用 MTT 细胞毒性测定法检测的牛精子活力在 NP 溶解在 0.1%ETOH 的所有实验组中均显著降低（P<0.001）。NP 溶解在 0.1%DMSO 的实验组中，NP 浓度为 1μg/ml 时活力显著降低（P<0.05），浓度>10μg/ml 时活力显著降低（P<0.001）。培养 6 h 后，MTT 试验证明了所有 NP 剂量对细胞活力的负面影响。研究结果清楚地表明 NP 作为一种内分泌干扰物对精子活力和活力的负面影响，在动物和人类接触 NP 时应予以高度重视，并作为男性生殖功能障碍的原因。
