Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Department of Resources and Environment, Baoshan University, Baoshan 678000, PR China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Biosens Bioelectron. 2015 Feb 15;64:416-22. doi: 10.1016/j.bios.2014.09.041. Epub 2014 Oct 2.
Herein, we present a novel electrochemical sensor for the determination of chloramphenicol (CAP), which is based on multiwalled carbon nanotubes@molecularly imprinted polymer (MWCNTs@MIP), mesoporous carbon (CKM-3) and three-dimensional porous graphene (P-r-GO). Firstly, 3-hexadecyl-1-vinylimidazolium chloride (C16VimCl) was synthetized by using 1-vinylimidazole and 1-chlorohexadecane as precursors. Then, C16VImCl was used to improve the dispersion of MWCNT and as monomer to prepare MIP on MWCNT surface to obtain MWCNTs@MIP. After that, the obtained MWCNTs@MIP was coated on the CKM-3 and P-r-GO modified glassy carbon electrode to construct an electrochemical sensor for the determination of CAP. The parameters concerning this assay strategy were carefully considered. Under the optimal conditions, the electrochemical sensor offered an excellent response for CAP. The linear response ranges were 5.0 × 10(-9)-5 × 10(-7)mol L(-1) and 5.0 × 10(-7)-4.0 × 10(-6), respectively, and the detection limit was 1.0 × 10(-10)mol L(-1). The electrochemical sensor was applied to determine CAP in real samples with satisfactory results.
本文提出了一种基于多壁碳纳米管@分子印迹聚合物（MWCNTs@MIP）、介孔碳（CKM-3）和三维多孔石墨烯（P-r-GO）的新型电化学传感器，用于测定氯霉素（CAP）。首先，通过使用 1-乙烯基咪唑和 1-氯十六烷作为前体合成了 3-十六烷基-1-乙烯基咪唑氯盐（C16VimCl）。然后，C16VImCl 被用于改善 MWCNT 的分散性，并作为单体在 MWCNT 表面制备 MIP，从而获得 MWCNTs@MIP。之后，将所得的 MWCNTs@MIP 涂覆在 CKM-3 和 P-r-GO 修饰的玻碳电极上，构建用于测定 CAP 的电化学传感器。仔细考虑了该分析策略的参数。在最佳条件下，电化学传感器对 CAP 表现出优异的响应。线性响应范围分别为 5.0×10(-9)-5×10(-7)mol L(-1)和 5.0×10(-7)-4.0×10(-6)，检测限为 1.0×10(-10)mol L(-1)。该电化学传感器已成功应用于实际样品中 CAP 的测定，结果令人满意。
