Razmi Habib, Maleki Fatemeh, Saedi Behnaz
Department of Chemistry, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, 53714-161, Iran.
Mikrochim Acta. 2025 Jul 21;192(8):506. doi: 10.1007/s00604-025-07329-0.
An electrochemical sensing platform was developed for the detection of tryptophan (Trp) using a molecularly imprinted polydopamine grafted with functionalized multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode (GCE), referred to as MWCNT-COOH/PDA-MIP. Dopamine, as a monomer, underwent self-polymerization in the presence of MWCNT-COOH and the target molecule, Trp, forming a polydopamine network. The synthesized composite was dispersed in an aqueous solvent and cast onto the GCE surface. Trp was then removed from the polymer matrix, creating molecularly imprinted cavities tailored to the size and properties of the target molecule. These cavities enabled the MWCNT-COOH/PDA-MIP to adsorb and recognize Trp with high precision and selectivity. This novel electrochemical sensor achieves an ultra-low detection limit and enhanced specificity for tryptophan detection through the integration of molecularly imprinted polydopamine with functionalized MWCNTs. The morphology and composition of the prepared sensor were analyzed by FT-IR, XRD, EDX, and SEM techniques. Sensor performance was evaluated using differential pulse voltammetry (DPV), revealing a linear response over two concentration ranges: 10 to 10 M and 10 to 10 M. The limit of detection (LOD) and limit of quantification (LOQ) were 4.33 × 10 M and 14.43 × 10 M, respectively. The sensor was successfully applied to measure Trp in real samples, including blood serum and milk, achieving satisfactory results with an average recovery of 97.15%.
开发了一种电化学传感平台，用于检测色氨酸（Trp），该平台使用接枝有功能化多壁碳纳米管（MWCNTs）的分子印迹聚多巴胺修饰玻碳电极（GCE），称为MWCNT-COOH/PDA-MIP。多巴胺作为单体，在MWCNT-COOH和目标分子Trp存在下进行自聚合，形成聚多巴胺网络。将合成的复合材料分散在水性溶剂中并浇铸到GCE表面。然后从聚合物基质中去除Trp，形成与目标分子的大小和性质相匹配的分子印迹空腔。这些空腔使MWCNT-COOH/PDA-MIP能够高精度和高选择性地吸附和识别Trp。这种新型电化学传感器通过将分子印迹聚多巴胺与功能化MWCNT集成，实现了超低检测限和增强的色氨酸检测特异性。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、能量色散X射线光谱（EDX）和扫描电子显微镜（SEM）技术分析了制备的传感器的形态和组成。使用差分脉冲伏安法（DPV）评估传感器性能，结果显示在两个浓度范围内呈线性响应：10至10 M和10至10 M。检测限（LOD）和定量限（LOQ）分别为4.33×10 M和14.43×10 M。该传感器成功应用于实际样品（包括血清和牛奶）中Trp的测定，获得了满意的结果，平均回收率为97.15%。
