Antunes Michelle S, Goes André T R, Boeira Silvana P, Prigol Marina, Jesse Cristiano R
Laboratório de avaliações farmacológicas e toxicológicas aplicadas às moléculas bioativas-LaftamBio Pampa-Universidade Federal do Pampa, Itaqui, RS, Brazil.
Laboratório de avaliações farmacológicas e toxicológicas aplicadas às moléculas bioativas-LaftamBio Pampa-Universidade Federal do Pampa, Itaqui, RS, Brazil.
Nutrition. 2014 Nov-Dec;30(11-12):1415-22. doi: 10.1016/j.nut.2014.03.024. Epub 2014 Apr 12.
Parkinson's disease (PD) may be caused by the interaction of a number of factors, including genetics, toxins, oxidative stress, mitochondrial abnormalities, and aging. Studies have shown that consumption of an antioxidant-rich diet may reduce the incidence of neurodegenerative diseases. The aim of this study was to evaluate the role of the flavonoid hesperidin in an animal model of PD induced by 6-hidroxidopamine (6-OHDA).
Aged mice were treated with hesperidin (50 mg/kg) during 28 d after an intracerebroventricular injection of 6-OHDA. The enzymatic activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and glutathione S-transferase, the levels of glutathione, reactive oxygen species, total reactive antioxidant potential, dopamine and its levels of metabolites 3,4-dihydroxyphenylacetic acid and homovanillic acid, was analyzed in the striatum. The behavioral parameters (depressive-like, memory, and locomotor) were measured.
This study demonstrated that hesperidin (50 mg/kg) treatment was effective in preventing memory impairment in the Morris water maze test, as well as, depressive-like behavior in the tail suspension test. Hesperidin attenuated the 6-OHDA-induced reduction in glutathione peroxidase and catalase activity, total reactive antioxidant potential and the dopamine and its metabolite levels in the striatum of aged mice. 6-OHDA increased reactive oxygen species levels and glutathione reductase activity in the striatum, and these alterations were mitigated by chronic administration of hesperidin.
This study demonstrated a protective effect of hesperidin on the neurotoxicity induced by 6-OHDA in aged mice, indicating that it could be useful as a therapy for the treatment of PD.
帕金森病（PD）可能由多种因素相互作用引起，包括遗传、毒素、氧化应激、线粒体异常和衰老。研究表明，食用富含抗氧化剂的饮食可能会降低神经退行性疾病的发病率。本研究的目的是评估类黄酮橙皮苷在6-羟基多巴胺（6-OHDA）诱导的PD动物模型中的作用。
老年小鼠在脑室内注射6-OHDA后28天内接受橙皮苷（50mg/kg）治疗。分析纹状体中超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶的酶活性、谷胱甘肽水平、活性氧、总活性抗氧化能力、多巴胺及其代谢产物3,4-二羟基苯乙酸和高香草酸的水平。测量行为参数（抑郁样、记忆和运动）。
本研究表明，橙皮苷（50mg/kg）治疗可有效预防莫里斯水迷宫试验中的记忆障碍以及悬尾试验中的抑郁样行为。橙皮苷减轻了6-OHDA诱导的老年小鼠纹状体中谷胱甘肽过氧化物酶和过氧化氢酶活性、总活性抗氧化能力以及多巴胺及其代谢产物水平的降低。6-OHDA增加了纹状体中的活性氧水平和谷胱甘肽还原酶活性，而长期给予橙皮苷可减轻这些改变。
本研究证明了橙皮苷对老年小鼠6-OHDA诱导的神经毒性具有保护作用，表明它可能作为治疗PD的一种疗法。
