National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues and ‡MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University , Wuhan, Hubei 430070, People's Republic of China.
J Agric Food Chem. 2014 Oct 22;62(42):10348-56. doi: 10.1021/jf5042867. Epub 2014 Oct 13.
A residue depletion study was performed to investigate the tissue kinetics of quinocetone (1) and its major metabolites. Quinocetone and its major metabolites were simultaneously quantitated with a high-performance liquid chromatography-ultraviolet (HPLC-UV) method. A total of 25 pigs, 30 broilers, and 50 carp were fed 100 mg/kg quinocetone for 90, 42, and 60 days, respectively. Liver, kidney, muscle, and fat (skin) tissues were collected at five different withdrawal times for analysis. Results revealed that quinocetone, 1-desoxyquinocetone (2), carbonyl-reduced 4-desoxyquinocetone (4), 3-methylquinoxaline-2-carboxylic acid (5), and carbonyl-reduced dideoxyquinocetone (6) could be depleted quickly in tissues; by contrast, dideoxyquinocetone, 3, persisted for a long time in the liver. Therefore, the liver is possibly the target tissue of quinocetone, and 3 is the residual marker; the recommended withdrawal times (WDTs) are 0 days in pigs and carp and 3 days in broilers. These results provided clear monitoring tools and technical standards to evaluate the food safety of quinocetone.
残留消除研究旨在研究喹乙醇（1）及其主要代谢物的组织动力学。采用高效液相色谱-紫外（HPLC-UV）法同时定量测定喹乙醇及其主要代谢物。共给 25 头猪、30 只肉鸡和 50 尾鲤鱼分别喂食 100mg/kg 的喹乙醇，持续 90、42 和 60 天。在五个不同的停药时间采集肝脏、肾脏、肌肉和脂肪（皮肤）组织进行分析。结果表明，喹乙醇、1-去氧喹乙醇（2）、羰基还原的 4-去氧喹乙醇（4）、3-甲基喹喔啉-2-羧酸（5）和羰基还原的二脱氧喹乙醇（6）可在组织中快速消除；相比之下，二脱氧喹乙醇、3-甲基喹喔啉-2-羧酸（5）和羰基还原的二脱氧喹乙醇（6）在肝脏中则持续存在很长时间。因此，肝脏可能是喹乙醇的靶组织，3 是残留标志物；推荐的停药时间（WDT）在猪和鲤鱼中为 0 天，在肉鸡中为 3 天。这些结果为评估喹乙醇的食品安全提供了明确的监测工具和技术标准。
