Bobeva Aneliya, Ilieva Mihaela, Dimitrov Dimitar, Zehtindjiev Pavel
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113, Sofia, Bulgaria,
Parasitol Res. 2014 Dec;113(12):4505-11. doi: 10.1007/s00436-014-4140-1. Epub 2014 Oct 4.
The occurrence of haemosporidians in biting midges of the genus Culicoides is examined in North-East Bulgaria in order to reveal their potential role for parasite transmission. A PCR-based technique amplifying part of the mitochondrial cytochrome b gene of the parasite is applied on naturally infected biting midges. Totally, 640 parous individuals of four species and 95 blood-fed individuals of six species of Culicoides are examined for the presence of DNA of haemosporidians. Haemosporidian genetic lineages are identified in individuals of three insect species: Culicoides alazanicus (12 lineages, nine lineages of Haemoproteus and three lineages of Plasmodium), Culicoides festivipennis and Culicoides circumscriptus (with two and one lineages of Haemoproteus, respectively). Two genetic lineages of Haemoproteus are recorded in more than one vector species. These results demonstrate variations in the specificity of Haemoproteus genetic lineages to their potential vectors, since some lineages are recorded in a single vector species and others occur in two or more vector species.
为揭示血孢子虫在寄生虫传播中的潜在作用，对保加利亚东北部库蠓属的吸血蠓中血孢子虫的出现情况进行了研究。一种基于聚合酶链反应（PCR）的技术，用于扩增寄生虫线粒体细胞色素b基因的部分序列，该技术应用于自然感染的吸血蠓。总共检测了库蠓属4个物种的640只已产卵个体和6个物种的95只吸血个体，以检测血孢子虫DNA的存在情况。在三种昆虫物种的个体中鉴定出血孢子虫遗传谱系：阿拉赞库蠓（12个谱系，其中9个变形血孢子虫谱系和3个疟原虫谱系）、节庆库蠓和环斑库蠓（分别有2个和1个变形血孢子虫谱系）。在不止一种媒介物种中记录到了两种变形血孢子虫遗传谱系。这些结果表明，变形血孢子虫遗传谱系对其潜在媒介的特异性存在差异，因为一些谱系只在单一媒介物种中记录到，而其他谱系则出现在两种或更多的媒介物种中。
