Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113, Sofia, Bulgaria,
Parasitol Res. 2013 Oct;112(10):3509-15. doi: 10.1007/s00436-013-3533-x. Epub 2013 Jul 14.
A total of 76 birds belonging to 23 species and 14 families was examined for the presence of Plasmodium spp. and Haemoproteus spp. Birds were trapped at four localities in Gansu Province, China, in June-July 2011. DNA was isolated from blood samples and parasite detection, and identification was based on PCR assays and sequences of 479 bp of cyt b gene. The total prevalence of haemosporidians was 21.0%. Haemoproteus spp. were detected in 14 birds (prevalence 18.4%). The lineage CYAPIC1 from Cyanopica cyanus, Parus major, Passer montanus and Pyrrhocorax pyrrhocorax was new; it is genetically distinct and probably represents a new species of the genus Haemoproteus. Three lineages represented known species: RBS4 (from Lanius tephronotus), a lineage of Haemoproteus lanii; COLL2 (from Turdus mupinensis), a lineage of Haemoproteus pallidus and TURDUS2 (from Turdus rubrocanus), a lineage of Haemoproteus minutus. The lineage RBS5 (from Lanius cristatus and L. tephronotus) differs by 1.4% from RBS4 and probably represents an intraspecific entity of H. lanii. The lineages TUCHR1 (recorded from T. mupinensis), WW1 (recorded from Upupa epops) and YWT2 (recorded from Motacilla flava) have not been linked to any known species for the moment. Only one bird was positive for Plasmodium (prevalence 1.4%), i.e. P. major infected with the lineage GRW4 of Plasmodium relictum. The latter lineage has been considered by previous studies as typical for migratory birds and having transmission in tropical areas only; its record in a sedentary bird in China suggests its transmission in temperate latitudes.
共检查了来自中国甘肃省四个地点的 23 种 14 科 76 只鸟，以检测疟原虫和血孢子虫属的存在。2011 年 6 月至 7 月期间，从血液样本中分离出 DNA 进行寄生虫检测，并基于 PCR 检测和细胞色素 b 基因 479 个核苷酸的序列进行鉴定。血孢子虫的总流行率为 21.0%。在 14 只鸟中检测到 Haemoproteus spp.（流行率 18.4%）。来自蓝矶鸫、大山雀、雀形目山雀和红腹锦鸡的 CYAPIC1 谱系是新的；它在遗传上是不同的，可能代表了 Haemoproteus 属的一个新种。三个谱系代表了已知的物种：来自白喉噪鹛的 RBS4（来自白喉噪鹛），Haemoproteus lanii 的一个谱系；来自红腹锦鸡的 COLL2（来自红腹锦鸡），Haemoproteus pallidus 的一个谱系和 TURDUS2（来自红腹锦鸡），Haemoproteus minutus 的一个谱系。与 RBS4 相比，来自红尾伯劳和白喉噪鹛的 RBS5 谱系差异为 1.4%，可能代表 H. lanii 的一个种内实体。来自白喉噪鹛的 TUCHR1（记录）、来自戴胜的 WW1（记录）和来自黄鹡鸰的 YWT2（记录）的谱系目前尚未与任何已知物种相关联。只有一只鸟对疟原虫呈阳性（流行率 1.4%），即感染疟原虫遗留物的大噪鹛。后者的谱系以前的研究认为是候鸟的典型特征，仅在热带地区传播；它在中国留鸟中的记录表明它在温带地区传播。
