Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol university, Bangkok, Thailand.
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol university, Bangkok, Thailand.
Clin Chim Acta. 2015 Jan 15;439:1-4. doi: 10.1016/j.cca.2014.09.027. Epub 2014 Oct 2.
The Sysmex UX-2000 is a new, fully automated integrated urine analyzer. This device analyzes all physical and chemical characteristics of urine and sediments in urine on single platform. Because sediment analysis by fluorescent flow cytometry has limited ability to classify some formed elements present in urine (e.g., casts), laboratories should develop criteria for manual microscopic examination of urinalysis following the use of the automated urine analyzer.
399 urine samples were collected from routine workload. All samples were analyzed on the automated analyzer and were then compared to the results of the manual microscopic method to establish optimal criteria. Another set of 599 samples was then used to validate the optimized criteria. The efficiency of criteria and review rate were calculated. The false-positive and false-negative cases were enumerated and clarified.
We can set 11 rules which are related to the parameters categorized by the UX-2000, including cells, casts, crystals, organisms, sperm, and flags. After optimizing the rules, the review rate was 54.1% and the false-negative rate was 2.8%.
The combination of both UX-2000 and manual microscopic method obtain the best results. The UX-2000 improves efficiency by reducing the time and labor associated with the specimen analysis process.
Sysmex UX-2000 是一种新型的、全自动的集成尿液分析仪器。该仪器可在单一平台上分析尿液和尿液沉淀物的所有物理和化学特性。由于荧光流式细胞术对尿液中某些形成的成分（例如管型）的分类能力有限，实验室应在使用自动化尿液分析仪后，制定尿液分析的手动显微镜检查标准。
从常规工作负荷中收集了 399 份尿液样本。所有样本均在自动分析仪上进行分析，然后与手动显微镜方法的结果进行比较，以确定最佳标准。然后使用另一组 599 个样本验证优化后的标准。计算了标准的效率和审核率。列举并澄清了假阳性和假阴性病例。
我们可以设置 11 个规则，这些规则与 UX-2000 分类的参数有关，包括细胞、管型、晶体、有机物、精子和标记。优化规则后，审核率为 54.1%，假阴性率为 2.8%。
将 UX-2000 与手动显微镜方法相结合可以获得最佳结果。UX-2000 通过减少与标本分析过程相关的时间和劳动力，提高了效率。
