Laiwejpithaya Sathima, Wongkrajang Preechaya, Reesukumal Kanit, Bucha Chonticha, Meepanya Suriya, Pattanavin Chanutchaya, Khejonnit Varanya, Chuntarut Achara
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Clin Lab Anal. 2018 Feb;32(2). doi: 10.1002/jcla.22249. Epub 2017 May 2.
Fully automated urine analyzers now play an important role in routine urinalysis in most laboratories. The recently introduced UriSed 3 has a new automated digital imaging urine sediment analyzer with a phase contrast feature. The aim of this study was to compare the performance of the UriSed 3 and UX-2000 automated urine sediment analyzers with each other and with the results of the manual microscopic method.
Two hundred seventy-seven (277) samples of leftover fresh urine from our hospital's central laboratory were evaluated by two automated urine sediment analyzers-UriSed 3 and UX-2000. The results of urine sediment analysis were compared between the two automated analyzers and against the results of the manual microscopic method.
Both devices demonstrated excellent agreement for quantitative measurement of red blood cells and white blood cells. UX-2000 had a lower coefficient correlation and demonstrated slightly lower agreement for squamous epithelial cells. Regarding semiquantitative analysis, both machines demonstrated very good concordance, with all applicable rates within one grade difference of the other machine. UriSed 3 had higher sensitivity for small round cells, while UX-2000 showed greater sensitivity for detecting bacteria and hyaline casts. UriSed 3 demonstrated slightly better specificity, especially in the detection of hyaline and pathological casts.
Both instruments had nearly similar performance for red blood cells and white blood cells measurement. UriSed 3 was more reliable for measuring squamous epithelial cells and small round cells, while the UX-2000 was more accurate for detecting bacteria and hyaline casts.
全自动尿液分析仪如今在大多数实验室的常规尿液分析中发挥着重要作用。最近推出的UriSed 3拥有一款具备相差功能的新型自动化数字成像尿沉渣分析仪。本研究的目的是比较UriSed 3和UX - 2000自动化尿沉渣分析仪彼此之间的性能，以及它们与手工显微镜检查法结果的差异。
使用UriSed 3和UX - 2000这两款自动化尿沉渣分析仪对我院中心实验室留存的277份新鲜尿液样本进行评估。比较这两款自动化分析仪的尿沉渣分析结果，并与手工显微镜检查法的结果进行对比。
两款设备在红细胞和白细胞的定量测量方面均显示出极佳的一致性。UX - 2000的系数相关性较低，在鳞状上皮细胞方面的一致性略低。在半定量分析方面，两台仪器的一致性都非常好，所有适用率在另一台仪器的一个等级差异范围内。UriSed 3对小圆细胞的敏感性较高，而UX - 2000在检测细菌和透明管型方面表现出更高的敏感性。UriSed 3的特异性略好，尤其是在透明管型和病理性管型的检测中。
两台仪器在红细胞和白细胞测量方面的性能几乎相似。UriSed 3在测量鳞状上皮细胞和小圆细胞方面更可靠，而UX - 2000在检测细菌和透明管型方面更准确。
