Chien Tzu-I, Kao Jau-Tsuen, Liu Hui-Lan, Lin Po-Chang, Hong Jhih-Sian, Hsieh Han-Peng, Chien Miao-Ju
Department of Laboratory Medicine, National Taiwan University Hospital, Taiwan.
Clin Chim Acta. 2007 Sep;384(1-2):28-34. doi: 10.1016/j.cca.2007.05.012. Epub 2007 May 26.
Several automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-100 and Iris iQ200 with manual microscopy in urine sediment testing.
Four hundred and thirty-six urine samples were collected. The urine sediments were examined by manual microscopy and these 2 automated urinalysis systems.
The within-run CVs for urine samples ranged from 3.4% to 22.3% for the iQ200, 1.6% to 24.2% for the UF-100 and 12.5% to 43.9% for manual microscopy. Between-run CVs on quality-control samples ranged from 6.1% to 32.4% for the iQ200 and 3.5% to 24.7% for the UF-100. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.935 to 0.968. However, for epithelial cells, the values measured by different systems were poorly correlated (r=0.888-0.922). The Bland-Altman plot indicated a trend towards the automated cell count being greater than the manual microscopy as the epithelial cell count increased. Casts were difficulty differentiated by 2 automated systems.
These 2 automated urinalysis systems demonstrated good concordance with each other in urine sediment examination. The automated process could be used as a screening procedure but some manual microscopy was still necessary.
已有多种检测尿沉渣的自动化仪器问世。我们比较了Sysmex UF - 100和Iris iQ200在尿沉渣检测中与手工显微镜检查的性能。
收集了436份尿液样本。通过手工显微镜检查以及这两种自动化尿液分析系统对尿沉渣进行检测。
iQ200对尿液样本的批内变异系数范围为3.4%至22.3%，UF - 100为1.6%至24.2%，手工显微镜检查为12.5%至43.9%。质量控制样本的批间变异系数，iQ200为6.1%至32.4%，UF - 100为3.5%至24.7%。基于0.935至0.968的r值，两种方法在红细胞和白细胞计数方面的一致性良好。然而，对于上皮细胞，不同系统测得的值相关性较差（r = 0.888 - 0.922）。Bland - Altman图表明，随着上皮细胞计数增加，自动化细胞计数有高于手工显微镜检查的趋势。管型在这两种自动化系统中难以区分。
这两种自动化尿液分析系统在尿沉渣检查中相互之间显示出良好的一致性。自动化检测过程可作为一种筛查程序，但仍需要一些手工显微镜检查。
