Chiliveri Sai Chaitanya, Deshmukh Mandar V
CSIR-Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad, 500007, Telangana, India.
Biomol NMR Assign. 2015 Oct;9(2):253-6. doi: 10.1007/s12104-014-9585-8. Epub 2014 Oct 4.
RNA interference (RNAi) is a conserved biological response to dsRNA and regulates the expression of protein-coding genes to mediate resistance to both endogenous parasitic and exogenous pathogenic nucleic acids. In RNAi pathway, dsRNA binding proteins assists Dicer at various stages of RNAi. In plants, DRB4, is a multidomain protein containing two dsRNA binding domains that recognizes the long exogenous/endogenous dsRNA and presents it to Ribonuclease enzyme, Dicer like 4, resulting in the production of 21 nt small interfering RNA. Here, we report nearly complete backbone and sidechain chemical shift assignments of N-terminus of DRB4 (1-153, ~18 kDa), containing both double stranded RNA binding domains and the linker.
RNA干扰（RNAi）是对双链RNA的一种保守生物学反应，可调节蛋白质编码基因的表达，以介导对内源寄生虫和外源致病核酸的抗性。在RNAi途径中，双链RNA结合蛋白在RNAi的各个阶段协助Dicer。在植物中，DRB4是一种多结构域蛋白，含有两个双链RNA结合结构域，可识别长的外源/内源双链RNA，并将其呈递给核糖核酸酶Dicer-like 4，从而产生21个核苷酸的小干扰RNA。在此，我们报告了DRB4（1-153，约18 kDa）N端几乎完整的主链和侧链化学位移归属，该N端包含双链RNA结合结构域和连接子。
