Paturi Sneha, Deshmukh Mandar V
CSIR - Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad, 500007, Telangana, India.
Biomol NMR Assign. 2023 Dec;17(2):173-178. doi: 10.1007/s12104-023-10137-3. Epub 2023 May 31.
In higher eukaryotes, the dsRNA binding proteins (dsRBPs) assist the corresponding Dicer in the cleavage of dsRNA precursors to effect post-transcriptional gene regulation through RNA interference. In contrast, the DRB7.2:DRB4 complex in Arabidopsis thaliana acts as a potent inhibitor of Dicer-like 3 (DCL3) processing by sequestering endogenous inverted-repeat dsRNA precursors. DRB7.2 possesses a single dsRNA Binding Domain (dsRBD) flanked by unstructured N- and C-terminal regions. Whereas, DRB4 has two concatenated N-terminal dsRBDs and a long unstructured C-terminus harboring a small domain of unidentified function, D3. Here, we present near-complete backbone and partial side chain assignments of the interaction domains, DRB7.2M (i.e., DRB7.2 (71-162)) and DRB4D3 (i.e., DRB4 (294-355)) as a complex. Our findings establish the groundwork for future structural, dynamic, and functional research on DRB7.2 and DRB4, and provide clues for the endo-IR pathway in plants.
在高等真核生物中，双链 RNA 结合蛋白（dsRBPs）协助相应的 Dicer 切割 dsRNA 前体，通过 RNA 干扰实现转录后基因调控。相比之下，拟南芥中的 DRB7.2:DRB4 复合物作为一种有效的 Dicer-like 3 (DCL3) 加工抑制剂，通过隔离内源性反向重复 dsRNA 前体发挥作用。DRB7.2 具有一个单双链 RNA 结合结构域（dsRBD），两侧为非结构化的 N-和 C-末端区域。然而，DRB4 具有两个串联的 N-末端 dsRBD 和一个长的非结构化 C-末端，其中包含一个功能未知的小结构域 D3。在这里，我们提出了相互作用结构域 DRB7.2M（即 DRB7.2（71-162））和 DRB4D3（即 DRB4（294-355））作为复合物的近完整骨架和部分侧链分配。我们的发现为 DRB7.2 和 DRB4 的未来结构、动态和功能研究奠定了基础，并为植物中的内源性 IR 途径提供了线索。
