鉴定表达淋巴管内皮透明质酸受体-1 的 bEnd.3 细胞的新特性。
Novel characterization of bEnd.3 cells that express lymphatic vessel endothelial hyaluronan receptor-1.
出版信息
Lymphology. 2014 Jun;47(2):73-81.
Murine bEnd.3 endothelioma cell line has been widely used in vascular research and here we report the novel finding that bEnd.3 cells express lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) and vascular endothelial growth factor receptor-3 (VEGFR-3). Moreover, these cells express progenitor cell markers of Sca-1 and CD133. Upon stimulation with tumor necrosis factor-alpha (TNF-alpha), the bEnd.3 cells demonstrate enhanced formation of capillary-type tubes, which express LYVE-1. As the bEnd.3 cell line is derived from murine endothelioma, we further examined human tissues of endothelioma and identified lymphatic vessels in the tumor samples which express both LYVE-1 and podoplanin. Moreover, a significantly higher number of lymphatic vessels were detected in the endothelioma samples compared with normal control. Taken together, this study not only redefines bEnd.3 cells for vascular research, but also indicates a broader category of human diseases that are associated with lymphatics, such as endothelioma.
鼠源性脑微血管内皮细胞系 bEnd.3 已被广泛应用于血管研究领域，在此我们报道了一个新发现，即 bEnd.3 细胞表达淋巴管内皮透明质酸受体-1（LYVE-1）和血管内皮生长因子受体-3（VEGFR-3）。此外，这些细胞还表达 Sca-1 和 CD133 等祖细胞标志物。在肿瘤坏死因子-α（TNF-α）的刺激下，bEnd.3 细胞表现出更强的毛细血管样管腔形成能力，并且表达 LYVE-1。由于 bEnd.3 细胞系源自鼠源性内皮瘤，我们进一步研究了人类内皮瘤组织，并在肿瘤样本中鉴定出既表达 LYVE-1 又表达 podoplanin 的淋巴管。此外，与正常对照组相比，内皮瘤样本中检测到的淋巴管数量明显更多。综上所述，本研究不仅重新定义了 bEnd.3 细胞在血管研究中的作用，还表明与淋巴管相关的更广泛的人类疾病类别，如内皮瘤。
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