Liu Ya-Li, Wang Ying, Yi Jia-Li, Jing Huan, Liu Chun-Ying
Zhongguo Zhong Yao Za Zhi. 2014 May;39(10):1869-73.
To explore the effect of Buzhong Yiqi decoction on PI3K/AKT signaling pathway in spleen, stomach and lung of nude mice with lung adenocarcinoma transplantation tumor.
Totally 60 nude mice were randomly divided into the blank control group, the tumor-bearing control group, the cisplatin group, the low-dose Buzhong Yiqi decoction group, the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group. After the corresponding interventions, efforts were made to measure the transplanted tumor volume and calculate the tumor inhibiting rate. The immunohistochemical method and real time PCR were used to detect the expression of PI3K and AKT level in nude mice spleen, stomach and lung.
Buzhong Yiqi decoction of different concentrations combined with cisplatin could inhibit the growth of the transplanted tumor, with the strongest inhibitory effect in the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group. All of the expressions of PI3K and AKT protein and gene in the spleen, stomach and lung increased, with the most significant increase in the tumor-bearing group. Along with the increase of the concentration of cisplatin and Buzhong Yiqi decoction, the expressions of PI3K and AKT gradually reduced. Compared with the tumor-bearing control group, there were statistical differences in spleen and stomach tissues (P < 0.05). Compared with the cisplatin group, the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group showed statistical differences (P < 0.05), but without statistical difference compared with the blank control group.
Among nude mice with lung adenocarcinoma transplantation tumor, the PI3K and AKT protein and gene expressions in spleen, stomach and lung tissues increased, which might indicated the effect of cisplatin and Buzhong Yiqi decoction in reducing PI3K and AKT expressions and the relations between the reduction degree and the concentrations of Buzhong Yiqi decoction. Cisplatin combined with Buzhong Yiqi decoction could decrease the PI3K and AKT protein and gene expression in spleen, stomach and lung, and make the pathway closer to normal, so as to protect the functions of spleen, stomach and lung, there may be target spots of Buzhong Yiqi decoction in PI3K/AKT signal pathway.
探讨补中益气汤对肺腺癌移植瘤裸鼠脾、胃、肺组织PI3K/AKT信号通路的影响。
将60只裸鼠随机分为空白对照组、荷瘤对照组、顺铂组、补中益气汤低剂量组、补中益气汤中剂量组、补中益气汤高剂量组。进行相应干预后，测量移植瘤体积并计算抑瘤率。采用免疫组化法和实时荧光定量PCR检测裸鼠脾、胃、肺组织中PI3K、AKT水平的表达。
不同浓度补中益气汤联合顺铂可抑制移植瘤生长，其中补中益气汤中剂量组和高剂量组抑制作用最强。脾、胃、肺组织中PI3K、AKT蛋白及基因表达均升高，荷瘤组升高最为显著。随着顺铂及补中益气汤浓度增加，PI3K、AKT表达逐渐降低。与荷瘤对照组比较，脾、胃组织差异有统计学意义（P<0.05）。与顺铂组比较，补中益气汤中剂量组和高剂量组差异有统计学意义（P<0.05），但与空白对照组比较差异无统计学意义。
肺腺癌移植瘤裸鼠脾、胃、肺组织中PI3K、AKT蛋白及基因表达升高，提示顺铂及补中益气汤有降低PI3K、AKT表达的作用，且降低程度与补中益气汤浓度有关。顺铂联合补中益气汤可降低脾、胃、肺组织中PI3K、AKT蛋白及基因表达，使该信号通路接近正常，从而保护脾、胃、肺功能，PI3K/AKT信号通路可能是补中益气汤的作用靶点。
