Yi Jia-li, Wang Ying, Jing Huan, Shi Yan-ting, Liu Chun-ying
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Oct;34(10):1250-5.
To analyze the reversal effect of Buzhong Yiqi Decoction (BYD) on multidrug resistance of human adenocarcinoma of lung cell line A549/DDP, and to study its effect on the expression of survivin by using serum pharmacological methods in vitro. Methods Totally 24 SD rats were divided into the high, medium and low dose groups, and the blank control group by randomized controlled method. The high dose BYD containing serum (1. 134 g/mL, 2 mL), the middle dose BYD containing serum (0.576 g/mL, 2 mL), and the low dose BYD containing serum (0.284 g/mL, 2 mL) were prepared. The inhibitory effects of different dose and concentrations BYD on the proliferation of A549 and A549/DDP cells were detected by MTT assay, and the drug resistance reversal fold was calculated. The expression of Survivin in the two cell strains were detected respectively by immunohistochemical assay, Western blot, and immunofluorescence method.
BYD containing serum showed obvious inhibitory effect on the growth of A549 and 549/DDP. The inhibition rates of 10% dose groups were higher than those of 5% dose groups. Besides, it gradually increased along with increased concentrations. Compared with 10% blank control group, the inhibition rate increased in 10% middle and low dose groups (P <0.05). After acted with 10% middle dose BYD containing serum, IC50, of A549 and A549/DDP were both reduced (P <0.05), reversal fold (RF) both increased. Its reversal ratio on A549/DDP cells was 2. 46, decreasing the resistance of A549/DDP to DDP. Compared with A549 in the same group, the expression of Survivin was detected to decrease by immunocytochemical assay, Western blot, and immunofluorescence method (P<0.05). Compared with 10% blank control group, the inhibition rate decreased in 10% middle dose group (P <0. 05).
10% middle dose BYD containing serum could significantly inhibit the apoptosis of A549 and A549/DDP. Besides, it could moderately reverse the multidrug resistance of A549/DDP cells to DDP possibly through reducing the intracellular expression of Survivin and enhancing the sensitivity 549/DDP to chemotherapeutics.
分析补中益气汤（BYD）对人肺腺癌细胞系A549/DDP多药耐药的逆转作用，并采用血清药理学方法体外研究其对生存素表达的影响。方法：将24只SD大鼠按随机对照法分为高、中、低剂量组和空白对照组。制备高剂量BYD含药血清（1.134 g/mL，2 mL）、中剂量BYD含药血清（0.576 g/mL，2 mL）和低剂量BYD含药血清（0.284 g/mL，2 mL）。采用MTT法检测不同剂量和浓度的BYD对A549和A549/DDP细胞增殖的抑制作用，并计算耐药逆转倍数。分别采用免疫组化法、Western blot法和免疫荧光法检测两种细胞株中生存素的表达。
BYD含药血清对A549和549/DDP的生长有明显抑制作用。10%剂量组的抑制率高于5%剂量组，且随浓度增加而逐渐升高。与10%空白对照组相比，10%中、低剂量组的抑制率升高（P<0.05）。10%中剂量BYD含药血清作用后，A549和A549/DDP的IC50均降低（P<0.05），逆转倍数（RF）均升高。其对A549/DDP细胞的逆转率为2.46，降低了A549/DDP对顺铂的耐药性。免疫组化法、Western blot法和免疫荧光法检测显示，与同组A549相比，生存素的表达降低（P<0.05）。与10%空白对照组相比，10%中剂量组的抑制率降低（P<0.05）。
10%中剂量BYD含药血清可显著抑制A549和A549/DDP的凋亡，还可能通过降低细胞内生存素的表达，增强549/DDP对化疗药物的敏感性，适度逆转A549/DDP细胞对顺铂的多药耐药性。
