Wu Liu-Hua, Lv Gui-Yuan, Li Bo, Zhang Yue-Li, Su Jie, Chen Su-Hong
Zhongguo Zhong Yao Za Zhi. 2014 May;39(10):1886-91.
To observe the effect of Polygonatum sibiricum on Yin deficiency model rats induced by long-term overload swimming.
Except for the normal group, all of the remaining rats performed the long-term overload swimming for eight weeks, with five days every week and once every day, to establish the Yin deficiency model. The daily swimming time increased from 10 min to 180 min at the end of the 7th week, with the water depth of 60 cm and the water temperature at 30 degrees C. After the success of the modeling, the rats were orally administered with different doses of aqueous extracts from P. sibiricum (2.5, 10 g x kg(-1)) for eight weeks. After the final administration, their blood were collected from orbits to measure immunoglobulin A, G and M (IgA, IgG, IgM), interleukin 2 and 6 (IL-2, IL-6) and cAMP, cGMP contents in plasma General behavioral indicators (weight, facial temperature, pain threshold and holding power) of rats were observed during the drug administration.
Compared with the model control group, aqueous extracts from P. sibiricum was given for eight weeks to significantly increase the rat weight and holding power of Yin deficiency model rats, decrease the facial temperature and the sensitivity of pain threshold, and increase IgA, IgG, IgM and IL-6 content and IgG content in serum, but without statistical difference. Aqueous extracts from P. sibiricum (10 g x kg(-1)) could also increase IL-2 content in serum, and decrease cAMP content and cAMP/cGMP ratio.
P. sibiricum could improve the general behavioral indicators (weight, holding power, pain threshold and facial temperature), immunologic functions (IgA, IgG, IgM) and cyclic nucleotide (cAMP, cAMP/cGMP), so as to ameliorate such Yin deficiency symptoms as dysphoria in chestpalms-soles, weight loss, soreness and weakness of waist and knees, immunologic dysfunction and cyclic nucleotide system disorders.
观察玉竹对长期超负荷游泳诱导的阴虚模型大鼠的影响。
除正常组外，其余大鼠均进行为期8周的长期超负荷游泳，每周5天，每天1次，以建立阴虚模型。第7周结束时，每日游泳时间从10分钟增加至180分钟，水深60厘米，水温30摄氏度。造模成功后，大鼠灌胃给予不同剂量的玉竹水提取物（2.5、10 g·kg⁻¹），持续8周。末次给药后，经眶静脉丛取血，检测血浆免疫球蛋白A、G、M（IgA、IgG、IgM）、白细胞介素2和6（IL-2、IL-6）以及环磷酸腺苷、环磷酸鸟苷含量。给药期间观察大鼠的一般行为指标（体重、面部温度、痛阈和握力）。
与模型对照组相比，给予玉竹水提取物8周可显著增加阴虚模型大鼠的体重和握力，降低面部温度和痛阈敏感性，增加血清中IgA、IgG、IgM和IL-6含量以及IgG含量，但无统计学差异。玉竹水提取物（10 g·kg⁻¹）还可增加血清中IL-2含量，降低环磷酸腺苷含量及环磷酸腺苷/环磷酸鸟苷比值。
玉竹可改善一般行为指标（体重、握力、痛阈和面部温度）、免疫功能（IgA、IgG、IgM）和环核苷酸（环磷酸腺苷、环磷酸腺苷/环磷酸鸟苷），从而改善诸如五心烦热、体重减轻、腰膝酸软、免疫功能紊乱和环核苷酸系统失调等阴虚症状。
