Ma Li-Na, Mao Xin-Min, Ma Xiao-Li, Li Lin-Lin, Wang Ye, Tao Yi-Cun, Wang Jing-Wei, Guo Jia-Jia, Lan Yi
Institute of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China.
Analytical&Testing Center of Xinjiang Medical University, Urumqi 830011, China.
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4226-4233. doi: 10.4268/cjcmm20162223.
To study the effect of plant protein and animal protein on amino acid metabolism spectrum of Qi and Yin deficiency type 2 diabetic rats. 110 male SD rats were randomly divided into blank group (n=10), diabetic model group (n=20), disease-symptoms group (n=80). The rats of blank group received ordinary feeding, while other groups were fed with high sugar and fat diets. During the whole process of feeding, rats of disease-symptoms group were given with Qingpi-Fuzi (15.75 g•kg⁻¹) once a day through oral administration. Five weeks later, the rats were given with a low dose of STZ (40 mg•kg⁻¹) by intraperitoneal injection to establish experimental diabetic models. Then the models were randomly divided into disease-symptoms group 1 (Qi and Yin deficiency diabetic group, 15.75 g•kg⁻¹), disease-symptoms group 2 (plant protein group, 0.5 g•kg⁻¹), disease-symptoms group 3 (animal protein group, 0.5 g•kg⁻¹), disease-symptoms group 4 (berberine group, 0.1 g•kg⁻¹). The drugs were given for 4 weeks by gavage administration. After 4 weeks of protein intervention, the abdominal aortic blood was collected and serum was isolated to analyze its free amino acid by using AQC pre-column derivatization HPLC and fluorescence detector. Four weeks after the protein intervention, plant protein, animal protein and berberine had no obvious effect on body weight and blood sugar in type 2 diabetic rats. As compared with animal protein group, histidine and proline（P<0.01）, serine, glycine, threonine, alanine, tyrosine, valine, methionine, bright+isoleucine, phenylalanine and lysine（P<0.05）changed a lot in rats serum of plant protein group.The results showed that gavage administration of protein would produce effects on amino acid metabolism of Qi and Yin deficiency type 2 diabetic SD rats. Symbolic differential compounds could be found through metabonomics technology, providing experimental basis for early warning of type 2 diabetes and diagnosis of Qi and Yin deficiency syndrome.
研究植物蛋白和动物蛋白对气阴两虚型2型糖尿病大鼠氨基酸代谢谱的影响。将110只雄性SD大鼠随机分为空白组（n = 10）、糖尿病模型组（n = 20）、造模组（n = 80）。空白组大鼠给予普通饲料喂养，其他组给予高糖高脂饲料喂养。在整个喂养过程中，造模组大鼠每天灌胃给予青皮-附子（15.75 g•kg⁻¹）。5周后，大鼠腹腔注射低剂量链脲佐菌素（STZ，40 mg•kg⁻¹）建立实验性糖尿病模型。然后将模型随机分为造模组1（气阴两虚糖尿病组，15.75 g•kg⁻¹）、造模组2（植物蛋白组，0.5 g•kg⁻¹）、造模组3（动物蛋白组，0.5 g•kg⁻¹）、造模组4（黄连素组，0.1 g•kg⁻¹）。药物灌胃给药4周。蛋白质干预4周后，采集腹主动脉血，分离血清，采用AQC柱前衍生化高效液相色谱-荧光检测法分析血清游离氨基酸。蛋白质干预4周后，植物蛋白、动物蛋白和黄连素对2型糖尿病大鼠体重和血糖无明显影响。与动物蛋白组相比，植物蛋白组大鼠血清中组氨酸和脯氨酸（P<0.01）、丝氨酸、甘氨酸、苏氨酸、丙氨酸、酪氨酸、缬氨酸、蛋氨酸、亮氨酸+异亮氨酸、苯丙氨酸和赖氨酸（P<0.05）变化较大。结果表明，灌胃给予蛋白质对气阴两虚型2型糖尿病SD大鼠氨基酸代谢有影响。通过代谢组学技术可发现标志性差异化合物，为2型糖尿病早期预警和气阴两虚证诊断提供实验依据。
