Lei Shan-shan, Lv Gui-yuan, Jin Ze-wu, Li Bo, Yang Zheng-biao, Chen Su-hong
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1793-7.
Some unhealthy life habits, such as long-term smoking, heavy drinking, sexual overstrain and frequent stay-up could induce the Yin deficiency symptoms of zygomatic red and dysphoria. Stems of Dendrobii officinalis flos (DOF) showed the efficacy of nourishing Yin. In this study, the hyperthyroidism Yin deficiency model was set up to study the yin nourishing effect and action mechanism of DOF, in order to provide the pharmacological basis for developing DOF resources and decreasing resource wastes. ICR mice were divided into five groups: the normal control group, the model control group, the positive control group and DOF extract groups (6.4 g · kg(-1)). Except for the normal group, the other groups were administrated with thyroxine for 30 d to set up the hyperthyroidism yin deficiency model. At the same time, the other groups were administrated with the corresponding drugs for 30 d. After administration for 4 weeks, the signs (facial temperature, pain domain, heart rate and autonomic activity) in mice were measured, and the facial and ear micro-circulation blood flow were detected by laser Doppler technology. After the last administration, all mice were fasted for 12 hours, blood were collected from their orbits, and serum were separated to detect AST, ALT, TG and TP by the automatic biochemistry analyzer and test T3, T4 and TSH levels by ELISA. (1) Compared with the normal control group, the model control group showed significant increases in facial and ear micro-circulation blood flow, facial temperature and heart rate (P < 0.05, P < 0.01), serum AST, ALT (P < 0.01), T3 level (P < 0.05), TSH level (P < 0.05) and notable deceases in pain domain (P < 0.01), TG level (P < 0.01). (2) Compared with the model control group, extracts from DOF (6 g · kg(-1)) could notably reduce facial and ear micro-circulation blood flow, facial temperature and heart rate (P < 0.05, P < 0.01) and AST (P < 0.05) and enhance pain domain (P < 0.01) and TG (P < 0.01). Extracts from DOF (4 g · kg(-1)) could remarkably reduce AST and ALT levels (P < 0.01, 0.05). Extracts from DOF (6 g · kg(-1) 4 g · kg(-1)) could significantly reduce T3 and increase serum TSH level (P < 0.05). DOF could improve Yin deficiency symptoms of zygomatic red and dysphoria in mice as well as liver function injury caused by overactive thyroid axis. According to its action mechanism, DOF may show yin nourishing and hepatic protective effects by impacting thyroxin substance metabolism, improving micro-circulation and reducing heart rate.
一些不良生活习惯，如长期吸烟、酗酒、房事过度和经常熬夜等，可引发颧红、烦躁等阴虚症状。铁皮石斛茎具有滋阴功效。本研究通过建立甲状腺功能亢进阴虚模型，探讨铁皮石斛茎的滋阴作用及作用机制，为开发铁皮石斛资源、减少资源浪费提供药理学依据。将ICR小鼠分为5组：正常对照组、模型对照组、阳性对照组和铁皮石斛提取物组（6.4 g·kg-1）。除正常组外，其余各组均给予甲状腺素30天以建立甲状腺功能亢进阴虚模型。同时，其余各组给予相应药物30天。给药4周后，测定小鼠体征（面部温度、痛域、心率和自主活动），采用激光多普勒技术检测面部和耳部微循环血流量。末次给药后，所有小鼠禁食12小时，摘眼球取血，分离血清，用自动生化分析仪检测AST、ALT、TG和TP，用ELISA法检测T3、T4和TSH水平。（1）与正常对照组比较，模型对照组面部和耳部微循环血流量、面部温度和心率显著升高（P<0.05，P<0.01），血清AST、ALT（P<0.01），T3水平（P<0.05），TSH水平（P<0.05），痛域显著降低（P<0.01），TG水平（P<0.01）。（2）与模型对照组比较，铁皮石斛提取物（6 g·kg-1）可显著降低面部和耳部微循环血流量、面部温度和心率（P<0.05，P<0.01）及AST（P<0.05），提高痛域（P<0.01）和TG（P<0.01）。铁皮石斛提取物（4 g·kg-1）可显著降低AST和ALT水平（P<0.01，0.05）。铁皮石斛提取物（6 g·kg-1、4 g·kg-1）可显著降低T3，提高血清TSH水平（P<0.05）。铁皮石斛可改善小鼠颧红、烦躁等阴虚症状以及甲状腺轴功能亢进所致肝功能损伤。从作用机制来看，铁皮石斛可能通过影响甲状腺素物质代谢、改善微循环、降低心率发挥滋阴和肝脏保护作用。
