Matthes Rutger, Assadian Ojan, Kramer Axel
Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, Germany.
Department for Hospital Hygiene and Infection Control, Medical University of Vienna, Vienna General Hospital, Vienna, Austria.
GMS Hyg Infect Control. 2014 Sep 30;9(3):Doc17. doi: 10.3205/dgkh000237. eCollection 2014.
The increasing microbial resistance against antibiotics complicates the therapy of bacterial infections. Therefore new therapeutic options, particularly those causing no resistance, are of high interest. Cold atmospheric plasma is one possible option to eradicate multidrug resistant microorganisms, and so far no resistance development against physical plasma is known.
We tested 6-fold repeated plasma applications on a Staphylococcus aureus strain embedded in biofilm and compared the reduction of the colony forming units between the different treatment periods to asses a possible development of resistance.
For all treatment periods, the control biofilms were reduced by plasma in average by 1.7 log10 CFU, and decreased from 7.6 to 5.8 log10 (CFU/cm(2)) within 5 hours. The results demonstrated that repeated plasma doses not induce resistance or habituation against plasma applied within short time periods.
The repeated application of cold plasma is a promising option for the treatment of infected wounds without the risk of development of resistance against plasma.
微生物对抗生素的耐药性不断增加，使细菌感染的治疗变得复杂。因此，新的治疗选择，特别是那些不会产生耐药性的选择，备受关注。冷大气等离子体是根除多重耐药微生物的一种可能选择，迄今为止，尚未发现对物理等离子体产生耐药性的情况。
我们对嵌入生物膜的金黄色葡萄球菌菌株进行了6次重复的等离子体处理，并比较了不同处理阶段菌落形成单位的减少情况，以评估是否可能产生耐药性。
在所有处理阶段，对照生物膜经等离子体处理后平均减少1.7个对数10 CFU，在5小时内从7.6降至5.8对数10（CFU/cm²）。结果表明，重复使用等离子体不会在短时间内诱导对等离子体的耐药性或适应性。
重复应用冷等离子体是治疗感染伤口的一个有前景的选择，不存在对等离子体产生耐药性的风险。
