Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Proteomics. 2011 Sep;11(17):3518-30. doi: 10.1002/pmic.201000637. Epub 2011 Aug 4.
Plasma medicine and also decontamination of bacteria with physical plasmas is a promising new field of life science with huge interest especially for medical applications. Despite numerous successful applications of low temperature gas plasmas in medicine and decontamination, the fundamental nature of the interactions between plasma and microorganisms is to a large extent unknown. A detailed knowledge of these interactions is essential for the development of new as well as for the enhancement of established plasma-treatment procedures. In the present work we introduce for the first time a growth chamber system suitable for low temperature gas plasma treatment of bacteria in liquid medium. We have coupled the use of this apparatus to a combined proteomic and transcriptomic analyses to investigate the specific stress response of Bacillus subtilis 168 cells to treatment with argon plasma. The treatment with three different discharge voltages revealed not only effects on growth, but also clear evidence of cellular stress responses. B. subtilis suffered severe cell wall stress, which was made visible also by electron microscopy, DNA damages and oxidative stress as a result of exposure to plasma. These biological findings were supported by the detection of reactive plasma species by OES measurements.
等离子体医学以及利用物理等离子体对细菌进行净化是生命科学中一个极有前途的新领域，尤其在医学应用方面引起了极大的关注。尽管低温气体等离子体在医学和净化方面有许多成功的应用，但等离子体与微生物之间相互作用的基本性质在很大程度上仍然未知。详细了解这些相互作用对于开发新的以及增强现有的等离子体处理程序都是至关重要的。在本工作中，我们首次引入了一种适合于在液体培养基中对细菌进行低温气体等离子体处理的生长室系统。我们将该设备的使用与联合蛋白质组学和转录组学分析相结合，以研究枯草芽孢杆菌 168 细胞对氩等离子体处理的特定应激反应。用三种不同的放电电压进行处理，不仅对生长有影响，而且还清楚地表明了细胞应激反应。枯草芽孢杆菌遭受严重的细胞壁应激，这一点也通过电子显微镜观察到，并且由于暴露于等离子体，还检测到 DNA 损伤和氧化应激。这些生物学发现得到了 OES 测量中检测到的反应性等离子体物质的支持。
