肝转移的临床前小鼠模型。
A preclinical murine model of hepatic metastases.
作者信息
Soares Kevin C, Foley Kelly, Olino Kelly, Leubner Ashley, Mayo Skye C, Jain Ajay, Jaffee Elizabeth, Schulick Richard D, Yoshimura Kiyoshi, Edil Barish, Zheng Lei
机构信息
Department of Surgery, The Johns Hopkins University School of Medicine.
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine.
出版信息
J Vis Exp. 2014 Sep 27(91):51677. doi: 10.3791/51677.
Abstract
Numerous murine models have been developed to study human cancers and advance the understanding of cancer treatment and development. Here, a preclinical, murine pancreatic tumor model of hepatic metastases via a hemispleen injection of syngeneic murine pancreatic tumor cells is described. This model mimics many of the clinical conditions in patients with metastatic disease to the liver. Mice consistently develop metastases in the liver allowing for investigation of the metastatic process, experimental therapy testing, and tumor immunology research.
摘要
已经开发了许多小鼠模型来研究人类癌症，并增进对癌症治疗和发展的理解。在此，描述了一种通过同种异体小鼠胰腺肿瘤细胞半脾注射建立肝转移的临床前小鼠胰腺肿瘤模型。该模型模拟了许多转移性疾病患者的临床情况。小鼠持续在肝脏中发生转移，从而可以对转移过程进行研究、进行实验性治疗测试以及开展肿瘤免疫学研究。
相似文献
1
A preclinical murine model of hepatic metastases.肝转移的临床前小鼠模型。
J Vis Exp. 2014 Sep 27(91):51677. doi: 10.3791/51677.
2
Description of a murine model of experimental hepatic metastases.实验性肝转移小鼠模型的描述。
J Natl Cancer Inst. 1986 Sep;77(3):823-8. doi: 10.1093/jnci/77.3.823.
3
Mechanism of liver-specific metastatic tumor spread in a murine tumor model.小鼠肿瘤模型中肝脏特异性转移性肿瘤扩散的机制
J Cancer Res Clin Oncol. 1988;114(1):47-58. doi: 10.1007/BF00390485.
4
Comparison of two syngeneic orthotopic murine models of pancreatic adenocarcinoma.两种胰腺腺癌同基因原位小鼠模型的比较。
J Invest Surg. 2013 Dec;26(6):352-9. doi: 10.3109/08941939.2013.797057. Epub 2013 Aug 19.
5
Consistent liver metastases in a rat model by portal injection of microencapsulated cancer cells.通过门静脉注射微囊化癌细胞在大鼠模型中产生持续性肝转移。
Cancer Res. 2006 Dec 1;66(23):11131-9. doi: 10.1158/0008-5472.CAN-06-0339.
6
[(111)In-DTPA]octreotide tumor uptake in GEPNET liver metastases after intra-arterial administration: an overview of preclinical and clinical observations and implications for tumor radiation dose after peptide radionuclide therapy.动脉内给药后[(111)铟-二巯基丁二酸]奥曲肽在GEPNET肝转移瘤中的摄取：临床前和临床观察概述以及肽受体放射性核素治疗后肿瘤辐射剂量的意义
Cancer Biother Radiopharm. 2014 May;29(4):179-87. doi: 10.1089/cbr.2013.1552.
7
8
In vivo imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model.在鼠原位胰腺癌模型和肝转移瘤模型中使用 7 特斯拉 MRI 进行胰腺肿瘤和肝转移瘤的体内成像。
BMC Cancer. 2011 Jan 28;11:40. doi: 10.1186/1471-2407-11-40.
9
Murine portal vein catheterization to analyze liver-directed therapies.建立小鼠门静脉插管以分析肝靶向治疗方法。
J Surg Res. 2013 Dec;185(2):690-6. doi: 10.1016/j.jss.2013.06.051. Epub 2013 Jul 17.
10
A novel approach to the generation and identification of experimental hepatic metastases in a murine model.一种在小鼠模型中生成和鉴定实验性肝转移的新方法。
J Natl Cancer Inst. 1986 Feb;76(2):309-22.
引用本文的文献
1
DHCR7 inhibition ameliorates MetALD and HCC in mice and human 3D liver spheroids.DHCR7抑制可改善小鼠和人3D肝球体中的代谢性脂肪性肝病和肝癌。
JHEP Rep. 2025 Apr 5;7(7):101415. doi: 10.1016/j.jhepr.2025.101415. eCollection 2025 Jul.
2
The scRNA-sequencing landscape of pancreatic ductal adenocarcinoma revealed distinct cell populations associated with tumor initiation and progression.胰腺导管腺癌的单细胞RNA测序图谱揭示了与肿瘤起始和进展相关的不同细胞群。
Genes Dis. 2024 May 10;12(3):101323. doi: 10.1016/j.gendis.2024.101323. eCollection 2025 May.
3
Reprogramming the Tolerogenic Immune Response Against Pancreatic Cancer Metastases by Lipid Nanoparticles Delivering a STING Agonist Plus Mutant KRAS mRNA.通过递送STING激动剂加突变型KRAS mRNA的脂质纳米颗粒重编程针对胰腺癌转移的耐受性免疫反应。
ACS Nano. 2025 Mar 11;19(9):8579-8594. doi: 10.1021/acsnano.4c14102. Epub 2025 Mar 2.
4
Enhancing Anti-PD-1 Immunotherapy by Targeting MDSCs via Hepatic Arterial Infusion in Breast Cancer Liver Metastases.通过肝动脉灌注靶向髓源性抑制细胞增强乳腺癌肝转移中的抗PD-1免疫疗法。
Cancers (Basel). 2024 Nov 3;16(21):3711. doi: 10.3390/cancers16213711.
5
Stromal reprogramming overcomes resistance to RAS-MAPK inhibition to improve pancreas cancer responses to cytotoxic and immune therapy.基质重编程克服了对 RAS-MAPK 抑制的抵抗，改善了胰腺癌细胞对细胞毒性和免疫治疗的反应。
Sci Transl Med. 2024 Oct 23;16(770):eado2402. doi: 10.1126/scitranslmed.ado2402.
6
Semaphorin 3D promotes pancreatic ductal adenocarcinoma progression and metastasis through macrophage reprogramming.信号蛋白 3D 通过重编程巨噬细胞促进胰腺导管腺癌的进展和转移。
Sci Adv. 2024 Oct 18;10(42):eadp0684. doi: 10.1126/sciadv.adp0684. Epub 2024 Oct 16.
7
IL15/IL15Rα complex induces an anti-tumor immune response following radiation therapy only in the absence of Tregs and fails to induce expansion of progenitor TCF1+ CD8 T cells.白细胞介素15/白细胞介素15受体α复合物仅在不存在调节性T细胞的情况下，放疗后诱导抗肿瘤免疫反应，且无法诱导祖细胞转录因子1阳性CD8 T细胞的扩增。
bioRxiv. 2024 Sep 22:2024.09.18.613691. doi: 10.1101/2024.09.18.613691.
8
Hypoxia-inducible factor 3α1 increases epithelial-to-mesenchymal transition and iron uptake to drive colorectal cancer liver metastasis.缺氧诱导因子3α1促进上皮-间质转化和铁摄取，从而驱动结直肠癌肝转移。
Br J Cancer. 2024 Jun;130(12):1904-1915. doi: 10.1038/s41416-024-02699-3. Epub 2024 May 1.
9
Mesothelin CAR T Cells Secreting Anti-FAP/Anti-CD3 Molecules Efficiently Target Pancreatic Adenocarcinoma and its Stroma.分泌抗-FAP/抗-CD3 分子的间皮素 CAR T 细胞可有效靶向胰腺腺癌及其基质。
Clin Cancer Res. 2024 May 1;30(9):1859-1877. doi: 10.1158/1078-0432.CCR-23-3841.
10
Chemokine Receptor 2 Targeted PET/CT Imaging Distant Metastases in Pancreatic Ductal Adenocarcinoma.趋化因子受体2靶向PET/CT成像检测胰腺导管腺癌远处转移
ACS Pharmacol Transl Sci. 2023 Dec 5;7(1):285-293. doi: 10.1021/acsptsci.3c00303. eCollection 2024 Jan 12.
本文引用的文献
1
EMT and dissemination precede pancreatic tumor formation. EMT 和播散先于胰腺肿瘤形成。
Cell. 2012 Jan 20;148(1-2):349-61. doi: 10.1016/j.cell.2011.11.025.
2
Tumor-associated antigen expressing Listeria monocytogenes induces effective primary and memory T-cell responses against hepatic colorectal cancer metastases.表达肿瘤相关抗原的李斯特菌单核细胞增生症诱导针对肝结直肠癌转移的有效原发性和记忆 T 细胞应答。
Ann Surg Oncol. 2012 Jul;19 Suppl 3(Suppl 3):S597-607. doi: 10.1245/s10434-011-2037-0. Epub 2011 Oct 7.
3
Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer.酪氨酸 23 磷酸化依赖性膜定位的膜联蛋白 A2 对于胰腺癌的侵袭和转移是必需的。
PLoS One. 2011 Apr 29;6(4):e19390. doi: 10.1371/journal.pone.0019390.
4
Effective depletion of regulatory T cells allows the recruitment of mesothelin-specific CD8 T cells to the antitumor immune response against a mesothelin-expressing mouse pancreatic adenocarcinoma.有效耗竭调节性 T 细胞可招募间皮素特异性 CD8 T 细胞参与抗肿瘤免疫反应，针对表达间皮素的小鼠胰腺腺癌。
Clin Transl Sci. 2008 Dec;1(3):228-39. doi: 10.1111/j.1752-8062.2008.00070.x.
5
Blood flow evaluation in high-frequency, 40 MHz imaging: a comparative study of four vector velocity estimation methods.高频（40MHz）成像中的血流评估：四种向量速度估计方法的比较研究。
Ultrasonics. 2010 Jun;50(7):683-90. doi: 10.1016/j.ultras.2010.01.008. Epub 2010 Jan 25.
6
Immunosurveillance of pancreatic adenocarcinoma: insights from genetically engineered mouse models of cancer.胰腺腺癌的免疫监视：来自癌症基因工程小鼠模型的见解。
Cancer Lett. 2009 Jun 28;279(1):1-7. doi: 10.1016/j.canlet.2008.09.037. Epub 2008 Nov 14.
7
Live attenuated Listeria monocytogenes effectively treats hepatic colorectal cancer metastases and is strongly enhanced by depletion of regulatory T cells.减毒活单核细胞增生李斯特菌可有效治疗肝转移性结直肠癌，并且通过清除调节性T细胞可显著增强其疗效。
Cancer Res. 2007 Oct 15;67(20):10058-66. doi: 10.1158/0008-5472.CAN-07-0573.
8
Maximizing mouse cancer models.优化小鼠癌症模型。
Nat Rev Cancer. 2007 Sep;7(9):645-58. doi: 10.1038/nrc2192.
9
Selective targeting of antitumor immune responses with engineered live-attenuated Listeria monocytogenes.利用工程减毒单核细胞增生李斯特菌对抗肿瘤免疫反应进行选择性靶向。
Cancer Res. 2006 Jan 15;66(2):1096-104. doi: 10.1158/0008-5472.CAN-05-2307.
10
Trp53R172H and KrasG12D cooperate to promote chromosomal instability and widely metastatic pancreatic ductal adenocarcinoma in mice.Trp53R172H和KrasG12D共同作用，促进小鼠染色体不稳定和广泛转移的胰腺导管腺癌。
Cancer Cell. 2005 May;7(5):469-83. doi: 10.1016/j.ccr.2005.04.023.
Zotero 插件