Soares Kevin C, Foley Kelly, Olino Kelly, Leubner Ashley, Mayo Skye C, Jain Ajay, Jaffee Elizabeth, Schulick Richard D, Yoshimura Kiyoshi, Edil Barish, Zheng Lei
Department of Surgery, The Johns Hopkins University School of Medicine.
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine.
J Vis Exp. 2014 Sep 27(91):51677. doi: 10.3791/51677.
Numerous murine models have been developed to study human cancers and advance the understanding of cancer treatment and development. Here, a preclinical, murine pancreatic tumor model of hepatic metastases via a hemispleen injection of syngeneic murine pancreatic tumor cells is described. This model mimics many of the clinical conditions in patients with metastatic disease to the liver. Mice consistently develop metastases in the liver allowing for investigation of the metastatic process, experimental therapy testing, and tumor immunology research.
已经开发了许多小鼠模型来研究人类癌症，并增进对癌症治疗和发展的理解。在此，描述了一种通过同种异体小鼠胰腺肿瘤细胞半脾注射建立肝转移的临床前小鼠胰腺肿瘤模型。该模型模拟了许多转移性疾病患者的临床情况。小鼠持续在肝脏中发生转移，从而可以对转移过程进行研究、进行实验性治疗测试以及开展肿瘤免疫学研究。
