Tatem Kathleen S, Quinn James L, Phadke Aditi, Yu Qing, Gordish-Dressman Heather, Nagaraju Kanneboyina
Center for Genetic Medicine Research, Children's National Medical Center;
Center for Genetic Medicine Research, Children's National Medical Center.
J Vis Exp. 2014 Sep 29(91):51785. doi: 10.3791/51785.
The open field activity monitoring system comprehensively assesses locomotor and behavioral activity levels of mice. It is a useful tool for assessing locomotive impairment in animal models of neuromuscular disease and efficacy of therapeutic drugs that may improve locomotion and/or muscle function. The open field activity measurement provides a different measure than muscle strength, which is commonly assessed by grip strength measurements. It can also show how drugs may affect other body systems as well when used with additional outcome measures. In addition, measures such as total distance traveled mirror the 6 min walk test, a clinical trial outcome measure. However, open field activity monitoring is also associated with significant challenges: Open field activity measurements vary according to animal strain, age, sex, and circadian rhythm. In addition, room temperature, humidity, lighting, noise, and even odor can affect assessment outcomes. Overall, this manuscript provides a well-tested and standardized open field activity SOP for preclinical trials in animal models of neuromuscular diseases. We provide a discussion of important considerations, typical results, data analysis, and detail the strengths and weaknesses of open field testing. In addition, we provide recommendations for optimal study design when using open field activity in a preclinical trial.
旷场活动监测系统全面评估小鼠的运动和行为活动水平。它是评估神经肌肉疾病动物模型中运动功能障碍以及可能改善运动和/或肌肉功能的治疗药物疗效的有用工具。旷场活动测量提供了一种与肌肉力量不同的测量方法，肌肉力量通常通过握力测量来评估。当与其他结果测量方法一起使用时，它还可以显示药物可能如何影响身体的其他系统。此外，总行进距离等测量方法类似于6分钟步行试验，这是一种临床试验结果测量方法。然而，旷场活动监测也面临重大挑战：旷场活动测量因动物品系、年龄、性别和昼夜节律而异。此外，室温、湿度、光照、噪音甚至气味都会影响评估结果。总体而言，本文提供了一种经过充分测试和标准化的旷场活动标准操作规程，用于神经肌肉疾病动物模型中的临床前试验。我们讨论了重要的注意事项、典型结果、数据分析，并详细阐述了旷场测试的优缺点。此外，我们还提供了在临床前试验中使用旷场活动时优化研究设计的建议。
