基于石墨烯量子点（GQDs）和金纳米粒子（AuNPs）的荧光共振能量转移（FRET）生物传感器，用于检测金黄色葡萄球菌 mecA 基因序列。
A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus.
机构信息
Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Department of Mechanical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
出版信息
Biosens Bioelectron. 2015 May 15;67:595-600. doi: 10.1016/j.bios.2014.09.059. Epub 2014 Sep 28.
In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor.
在这项工作中，开发了一种基于石墨烯量子点（GQDs）和金纳米粒子（AuNPs）对的新型荧光共振能量转移（FRET）生物传感器，用于检测金黄色葡萄球菌特定基因序列。该 FRET 生物传感器平台通过将捕获探针固定在 GQDs 上和将报告探针缀合在 AuNPs 上来实现。目标寡核苷酸然后与捕获探针和报告探针共杂交形成三明治结构，使 GQDs 和 AuNPs 接近以触发 FRET 效应。测量加入目标前后的荧光信号，并且在 100 nM 目标寡核苷酸的情况下，荧光猝灭效率可达到约 87%。该 FRET 生物传感器对金黄色葡萄球菌基因检测的检测限（LOD）约为 1 nM。使用单碱基错配寡核苷酸和双碱基错配寡核苷酸的实验证明了该 FRET 生物传感器具有良好的序列选择性。
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