基于石墨烯量子点和芘功能化分子信标的通用荧光生物传感器平台用于检测微小RNA
Universal Fluorescence Biosensor Platform Based on Graphene Quantum Dots and Pyrene-Functionalized Molecular Beacons for Detection of MicroRNAs.
作者信息
Zhang Huan, Wang Yunsheng, Zhao Daiwei, Zeng Dongdong, Xia Jiaoyun, Aldalbahi Ali, Wang Chenguang, San Lili, Fan Chunhai, Zuo Xiaolei, Mi Xianqiang
机构信息
‡Laboratory of System Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
§Thyroid Surgery, Second Affiliated Hospital of Guizhou Medical University, Kaili 556000, China.
出版信息
ACS Appl Mater Interfaces. 2015 Aug 5;7(30):16152-6. doi: 10.1021/acsami.5b04773. Epub 2015 Jul 27.
A novel biosensor platform was developed for detection of microRNAs (miRNAs) based on graphene quantum dots (GQDs) and pyrene-functionalized molecular beacon probes (py-MBs). Pyrene was introduced to trigger specifically fluorescence resonance energy transfer (FRET) between GQDs and fluorescent dyes labeled on py-MBs, and the unique fluorescent intensity change produced a novel signal for detection of the target. The platform realized detection of miRNAs in a wide range from 0.1 nM to 200 nM with great discrimination abilities, as well as multidetection of different kinds of miRNAs, which paved a brand new way for miRNA detection based on GQDs.
基于石墨烯量子点（GQDs）和芘功能化分子信标探针（py-MBs）开发了一种用于检测微小RNA（miRNAs）的新型生物传感器平台。引入芘以触发GQDs与py-MBs上标记的荧光染料之间的特异性荧光共振能量转移（FRET），独特的荧光强度变化产生用于检测靶标的新信号。该平台实现了在0.1 nM至200 nM的宽范围内对miRNAs的检测，具有很强的区分能力，以及对不同种类miRNAs的多重检测，为基于GQDs的miRNA检测开辟了一条全新的途径。
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