Jeong Won Je, Kim Jeong Yun, Choo Jaebum, Lee Eun Kyu, Han Chang Soo, Beebe David J, Seong Gi Hun, Lee Sang Hoon
Langmuir. 2005 Apr 26;21(9):3738-41. doi: 10.1021/la050105l.
We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and hydrogel solutions form emulsions in pressure-driven flow in microchannels at high values of the dimensionless capillary number (Ca). The resultant hydrogel droplets are then polymerized in situ via exposure to 365 nm UV light. This technique allows for the generation of monodisperse particles whose size can be controlled by the regulation of flow rates. In addition, both manufacturing microparticles and immobilizing biocatalysts can be performed simultaneously and continuously.
我们报道了一种通过微流体系统中不混溶液体的行为和原位光聚合来制造含有生物催化剂的聚合物微粒的新技术。该方法利用了可紫外光聚合的水凝胶/酶溶液和一种不混溶的油溶液。在微通道中压力驱动的流动中，油溶液和水凝胶溶液在无量纲毛细管数（Ca）较高时形成乳液。然后通过暴露于365nm紫外光使所得的水凝胶液滴原位聚合。该技术能够生成尺寸可通过流速调节来控制的单分散颗粒。此外，制造微粒和固定生物催化剂这两个过程可以同时且连续地进行。
