Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration, Iksan 570-080, Korea.
Plant Pathol J. 2013 Dec;29(4):446-50. doi: 10.5423/PPJ.NT.07.2013.0068.
The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.
禾谷镰刀菌是一种重要的麦类赤霉病病原菌，可产生多种真菌毒素，如单端孢霉烯族化合物和玉米赤霉烯酮。从空气中或谷物中分离真菌菌株可能会受到各种其他空气传播的真菌病原体和腐生真菌的阻碍。在这项研究中，我们使用细菌病原体伯克霍尔德菌产生的毒黄素开发了一种针对禾谷镰刀菌的选择性培养基。禾谷镰刀菌对毒黄素具有抗性，而其他真菌对这种毒素敏感。在培养基中添加毒黄素可以通过抑制腐生真菌的生长来增强对水稻粒中禾谷镰刀菌的分离。此外，在小麦田中暴露 1 小时的含或不含毒黄素的培养基分别有 84%或 25%的菌落被鉴定为禾谷镰刀菌。这种选择培养基为分离禾谷镰刀菌提供了一种有效的工具，并且可以被从事遗传学和疾病预测研究的小组采用。
