National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Korea.
Plant Pathol J. 2013 Dec;29(4):465-70. doi: 10.5423/PPJ.OA.05.2013.0048.
In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, and through sequencing 12 Pet-Stol isolates were identified. Comparison of the nucleotide sequences of 16S rRNA gene of the 12 Pet-Stol isolates with other phytoplasmas belonging to aster yellows or Stolbur showed that Pet-Stol isolates were members of Stolbur. The presence of phytoplasma in petunia was also confirmed by microscopic observation of the pathogens. In this study, Stolbur phytoplasma was identified from spreading type petunia cultivars by sequence analysis of 16S rRNA gene of phytoplasma and microscopic observation of phytoplasma bodies. This is the first report of Stolbur phytoplasma in commercial Petunia hybrida cultivars.
2012 年 1 月，从韩国京畿道牙山温室中收集到表现出异常生长习性的蔓延型矮牵牛品种“Wave Pink”，其侧芽萌发了多个异常的小植株。使用引物对 P1/P6 和 R16F1/R1 进行巢式 PCR，调查是否存在植原体。在巢式 PCR 中，获得了 1096 bp 的 PCR 产物，并通过测序鉴定了 12 个 Pet-Stol 分离株。将 12 个 Pet-Stol 分离株的 16S rRNA 基因的核苷酸序列与属于 Aster yellows 或 Stolbur 的其他植原体进行比较，结果表明 Pet-Stol 分离株属于 Stolbur。通过植原体体的显微镜观察也证实了矮牵牛中的植原体的存在。在这项研究中，通过植原体 16S rRNA 基因的序列分析和植原体体的显微镜观察，从蔓延型矮牵牛品种中鉴定出了 Stolbur 植原体。这是首例关于商业矮牵牛品种的 Stolbur 植原体的报道。
