Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
The Huck Institutes of the Life Sciences, Center for Infectious Disease Dynamics, Millennium Science Complex, The Pennsylvania State University, University Park, PA 16802, USA.
Plant Pathol J. 2014 Jun;30(2):151-8. doi: 10.5423/PPJ.OA.01.2014.0005.
Three double-stranded RNAs (dsRNAs), approximately 1.85, 1.65 and 1.27 kb in size, were detected in an isolate of Cytospora sacchari from Iran. Partial nucleotide sequence revealed a 1,284 bp segment containing one ORF that potentially encodes a 405 aa protein. This protein contains conserved motifs related to RNA dependent RNA polymerases (RdRp) that showed similarity to RdRps of partitiviruses. The results indicate that these dsRNAs represent a novel Partitivirus that we tentatively designate Cytospora sacchari partitivirus (CsPV). Treatment of the fungal strain by cyclohexamide and also hyphal tip culture had no effect on removing the putative virus. Phylogenetic analysis of putative RdRp of CsPV and other partitiviruses places CsPV as a member of the genus Partitivirus in the family Partitiviridae, and clustering with Aspergillus ochraceous virus 1.
从伊朗的 Cytospora sacchari 分离物中检测到 3 个双链 RNA（dsRNA），大小约为 1.85、1.65 和 1.27 kb。部分核苷酸序列揭示了一个包含一个可能编码 405 个氨基酸的 ORF 的 1,284 个碱基对片段。该蛋白含有与 RNA 依赖性 RNA 聚合酶（RdRp）相关的保守基序，与部分病毒的 RdRp 具有相似性。结果表明，这些 dsRNA 代表一种新型的 Partitivirus，我们暂定命名为 Cytospora sacchari partitivirus（CsPV）。用环己酰胺处理真菌菌株和菌丝尖端培养对去除假定的病毒没有影响。CsPV 的假定 RdRp 和其他部分病毒的系统发育分析将 CsPV 归类为 Partitiviridae 科的 Partitivirus 属成员，并与 Aspergillus ochraceous virus 1 聚类。
