Jiang Yinhui, Wang Jingxian, Yang Bi, Wang Qinrong, Zhou Jianjiang, Yu Wenfeng
Key Laboratory of Endemic and Ethnic Diseases, Guizhou Medical University, Ministry of Education, Guiyang, China.
Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Guiyang, China.
Front Microbiol. 2019 Mar 28;10:626. doi: 10.3389/fmicb.2019.00626. eCollection 2019.
The opportunistic human pathogenic fungus is known to be infected with mycoviruses. In this study, we report a novel mycovirus partitivirus 1 (AfPV1) that was originally isolated from the abnormal colonial morphology isolate LD-3-8 of . AfPV1 has spherical virus-like particles about 40 nm in diameter, and three double-stranded RNA (dsRNA) segments (dsRNA1, 2, and 3 with lengths of 1.7, 1.4, and 1.1 kbp, respectively) were packaged in the virions. dsRNA1, dsRNA2, and dsRNA3 each contained a single open reading frame and potentially encoded 62, 42, and 32 kDa proteins, respectively. The dsRNA1 encoded protein shows similarity to the RNA-dependent RNA polymerase (RdRp) of partitiviruses, and the dsRNA2 product has no significant similarity to any other capsid protein (CP) in the GenBank databases, beside some homology with the hypothetical "capsid" protein of a few partitiviruses. The dsRNA3 encodes a protein with no similarity to any protein in the GenBank database. SDS-PAGE and polypeptide mass fingerprint-mass spectrum (PMF-MS) analyses indicated that the CP of the AfPV1 was encoded by dsRNA2. Phylogenetic analysis showed that the AfPV1 and relative viruses were found in an unclassified group inside the family. AfPV1 seems to result in debilitation symptoms, but had no significant effects to murine pathogenicity. These findings provide new insights into the partitiviruses taxonomy and the interactions between viruses and .
已知机会性人类致病真菌会感染真菌病毒。在本研究中，我们报告了一种新型真菌病毒——分病毒1（AfPV1），它最初是从异常菌落形态分离株LD - 3 - 8中分离出来的。AfPV1具有直径约40 nm的球形病毒样颗粒，病毒粒子中包裹着三个双链RNA（dsRNA）片段（dsRNA1、dsRNA2和dsRNA3，长度分别为1.7、1.4和1.1 kbp）。dsRNA1、dsRNA2和dsRNA3各自包含一个单一的开放阅读框，分别可能编码62 kDa、42 kDa和32 kDa的蛋白质。dsRNA1编码的蛋白质与分病毒的RNA依赖RNA聚合酶（RdRp）相似，dsRNA2产物与GenBank数据库中的任何其他衣壳蛋白（CP）均无显著相似性，仅与少数分病毒的假定“衣壳”蛋白有一些同源性。dsRNA3编码的蛋白质与GenBank数据库中的任何蛋白质均无相似性。SDS - PAGE和多肽质量指纹 - 质谱（PMF - MS）分析表明，AfPV1的CP由dsRNA2编码。系统发育分析表明，AfPV1和相关病毒在该科内的一个未分类组中被发现。AfPV1似乎会导致衰弱症状，但对小鼠致病性没有显著影响。这些发现为分病毒分类学以及病毒与该真菌之间的相互作用提供了新的见解。
