Lv Huicheng, Guo Jun, Li Siqin, Jiang Dianmin
Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 404000, P.R. China ; Department of Orthopaedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China.
Department of Orthopaedics, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010030, P.R. China.
Exp Ther Med. 2014 Nov;8(5):1575-1580. doi: 10.3892/etm.2014.1942. Epub 2014 Sep 1.
As the most common malignant primary bone tumor in childhood, osteosarcoma (OS) maintains a high recurrence, despite the significant improvements in the overall survival rate of high-grade OS patients during the recent decades. Therefore, a novel therapy strategy is required for OS treatment. Recently, various microRNAs (miRNAs or miRs) have been confirmed as deregulated in OS, and the dysregulation in OS has been discovered by the microarray analysis. In the present study, the regulation of on the OS cell proliferation, migration and invasion on the MG-63 cells was explored . The mimics were found to promote cell proliferation, colony formation, migration and invasion significantly, compared to the control miRNA. An inhibitor was also used to evaluate whether served as a therapeutic target for OS. The results demonstrated that the inhibitor significantly reduced the proliferation, colony formation, migration and invasion of the MG-63 OS cells. Thus, the study confirmed the oncogenic regulation on the OS progression of , which could serve as a therapeutic target with an inhibitor.
骨肉瘤（OS）作为儿童期最常见的原发性恶性骨肿瘤，尽管近几十年来高级别OS患者的总体生存率有了显著提高，但仍保持着较高的复发率。因此，OS治疗需要一种新的治疗策略。最近，各种微小RNA（miRNA或miR）已被证实在OS中失调，并且通过微阵列分析发现了OS中的这种失调。在本研究中，探讨了[具体miRNA名称未给出]对MG-63细胞中OS细胞增殖、迁移和侵袭的调控作用。与对照miRNA相比，发现[具体miRNA名称未给出]模拟物可显著促进细胞增殖、集落形成、迁移和侵袭。还使用了[具体miRNA名称未给出]抑制剂来评估[具体miRNA名称未给出]是否可作为OS的治疗靶点。结果表明，[具体miRNA名称未给出]抑制剂显著降低了MG-63 OS细胞的增殖、集落形成、迁移和侵袭。因此，该研究证实了[具体miRNA名称未给出]对OS进展的致癌调控作用，其可作为使用[具体miRNA名称未给出]抑制剂的治疗靶点。
