Isaksson A, Hultberg B
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Clin Chim Acta. 1989 Aug 15;183(2):155-62. doi: 10.1016/0009-8981(89)90331-8.
Enzyme immunoassay (EIA) methods with monoclonal antibodies specific for beta-hexosaminidase (Hex) isoenzymes A and B in human serum are presented. The proportion of Hex A obtained with the EIA-methods was similar to that found with ion-exchange chromatography and isoelectric focusing (about 60% of total Hex activity). The EIA-method for Hex B reacted to a similar extent with Hex B, Hex P and intermediate forms. A highly significant correlation was obtained between total Hex activity in human sera assayed with a conventional enzyme substrate method and the total Hex activity obtained as the sum of Hex A and Hex B analyzed with the EIA-methods. In sera from individuals with increased total activity of Hex there was a positive correlation between total activity of Hex, and percent of Hex B found with the EIA-methods. Investigation of sera from pregnant women and sera from patients with liver cirrhosis and cholestasis showed that the increase in total activity in these patient groups was mainly due to an increase of the isoenzyme forms reacting with the EIA-method for Hex B.
本文介绍了用人血清中针对β-己糖胺酶（Hex）同工酶A和B的单克隆抗体的酶免疫测定（EIA）方法。通过EIA方法获得的Hex A比例与离子交换色谱法和等电聚焦法得到的比例相似（约占总Hex活性的60%）。Hex B的EIA方法与Hex B、Hex P和中间形式的反应程度相似。用传统酶底物法测定的人血清中总Hex活性与用EIA方法分析得到的Hex A和Hex B之和的总Hex活性之间存在高度显著的相关性。在Hex总活性增加的个体血清中，Hex总活性与用EIA方法测得的Hex B百分比之间呈正相关。对孕妇血清以及肝硬化和胆汁淤积患者血清的研究表明，这些患者组中总活性的增加主要是由于与Hex B的EIA方法反应的同工酶形式增加。
