Hultberg B, Isaksson A, Lindgren A, Israelsson B, Brattström L
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Ann Clin Biochem. 1996 Sep;33 ( Pt 5):432-7. doi: 10.1177/000456329603300506.
Plasma/serum beta-hexosaminidase (Hex) activity is known to be increased in chronic alcoholism, liver disorders, pregnancy and diabetes mellitus. Hex activity also shows an association with risk factors for vascular disease and heredity for arteriosclerosis. There are several isoenzymes of Hex. Using an enzyme immunoassay for Hex isoenzymes (Hex A and Hex B) we studied possible determinants of Hex isoenzymes and their relation to vascular disease in randomly invited (n = 244) 35-95-year-old men and women. In both sexes there were significant age-related increases in Hex activities and men exhibited higher activity of both isoenzymes. Both Hex isoenzymes correlated with age, systolic blood pressure, serum triglycerides and liver enzymes, whereas Hex A was distinguished from Hex B by its stronger correlation with blood glucose. In multiple linear regression analysis Hex A was explained to 20.7% by blood glucose, age, serum aspartate aminotransferase and glutamyl transpeptidase. Hex B was explained to 14% by age, serum glutamyl transpeptidase and serum triglycerides. There was no significant increase in Hex isoenzymes in subjects with hypertension, diabetes mellitus or myocardial disease, nor did current smokers exhibit any increase of these enzymes compared to non-smokers. The main conclusion in that liver function, as reflected by the level of liver enzymes and glucose metabolism, is the major determinant for Hex isoenzymes in plasma.
已知在慢性酒精中毒、肝脏疾病、妊娠和糖尿病患者中，血浆/血清β - 己糖胺酶（Hex）活性会升高。Hex活性还与血管疾病的危险因素和动脉硬化的遗传因素有关。Hex有几种同工酶。我们采用酶免疫分析法检测Hex同工酶（Hex A和Hex B），对随机邀请的244名35 - 95岁的男性和女性进行研究，以探讨Hex同工酶的可能决定因素及其与血管疾病的关系。在男性和女性中，Hex活性均随年龄显著增加，且男性的两种同工酶活性均较高。两种Hex同工酶均与年龄、收缩压、血清甘油三酯和肝酶相关，而Hex A与血糖的相关性更强，这使其与Hex B有所区别。在多元线性回归分析中，血糖、年龄、血清天冬氨酸转氨酶和谷氨酰转肽酶可解释Hex A变异的20.7%。年龄、血清谷氨酰转肽酶和血清甘油三酯可解释Hex B变异的14%。高血压、糖尿病或心肌病患者的Hex同工酶无显著升高，与不吸烟者相比，目前吸烟者的这些酶也未出现升高。主要结论是，肝酶水平和葡萄糖代谢所反映的肝功能是血浆中Hex同工酶的主要决定因素。
