Rosillo María Ángeles, Alcaraz María José, Sánchez-Hidalgo Marina, Fernández-Bolaños José G, Alarcón-de-la-Lastra Catalina, Ferrándiz María Luisa
Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.
Department of Pharmacology and IDM, University of Valencia, 46100 Burjasot, Valencia, Spain.
J Nutr Biochem. 2014 Dec;25(12):1275-81. doi: 10.1016/j.jnutbio.2014.07.006. Epub 2014 Sep 16.
The consumption of extra virgin olive oil (EVOO) in Mediterranean countries has shown beneficial effects. A wide range of evidence indicates that phenolic compounds present in EVOO are endowed with anti-inflammatory properties. In this work, we evaluated the effects of EVOO-polyphenol extract (PE) in a model of rheumatoid arthritis, the collagen-induced arthritis model in mice. On day 0, DBA-1/J mice were immunized with bovine type II collagen. On day 21, mice received a booster injection. PE (100 and 200 mg/kg) was orally administered once a day from days 29 to 41 to arthritic mice. We have demonstrated that PE decreases joint edema, cell migration, cartilage degradation and bone erosion. PE significantly reduced the levels of proinflammatory cytokines and prostaglandin E2 in the joint as well as the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1. Our data indicate that PE inhibits c-Jun N-terminal kinase, p38 and signal transducer and activator of transcription-3. In addition, PE decreases nuclear factor κB translocation leading to the down-regulation of the arthritic process. These results support the interest of natural diet components in the development of therapeutic products for arthritic conditions.
地中海国家食用特级初榨橄榄油（EVOO）已显示出有益效果。大量证据表明，EVOO中存在的酚类化合物具有抗炎特性。在这项研究中，我们评估了EVOO-多酚提取物（PE）在类风湿性关节炎模型（小鼠胶原诱导性关节炎模型）中的作用。在第0天，用牛II型胶原免疫DBA-1/J小鼠。在第21天，小鼠接受加强注射。从第29天至41天，每天一次给关节炎小鼠口服PE（100和200mg/kg）。我们已经证明，PE可减轻关节水肿、细胞迁移、软骨降解和骨侵蚀。PE显著降低关节中促炎细胞因子和前列腺素E2的水平以及环氧合酶-2和微粒体前列腺素E合酶-1的表达。我们的数据表明，PE抑制c-Jun氨基末端激酶、p38和信号转导子及转录激活子-3。此外，PE减少核因子κB易位，导致关节炎进程下调。这些结果支持天然饮食成分在开发关节炎治疗产品方面的价值。
