Department of Orthopedics and Traumatology, University of Health Sciences, Adana City Training and Research Hospital, Adana, Türkiye.
Department of Orthopedics and Traumatology, Çukurova University Faculty of Medicine, Adana, Türkiye.
J Orthop Surg Res. 2024 Jun 8;19(1):338. doi: 10.1186/s13018-024-04818-5.
We aimed to analyze the outcomes of intraarticular extra virgin olive oil (EVOO) injection on mechanically induced rabbit knee osteoarthritis (OA) by studying the morphological, histological, and radiological findings.
The study was conducted on 32 New Zealand White rabbits. The randomly numbered subjects were divided into two main groups. The rabbits numbered 1 to 16 were selected to be the group to receive EVOO, and the remaining were selected into a control group. Both groups were separated into two subgroups for short-term (five weeks) and long-term (10 weeks) follow-up. Anterior cruciate ligament transection was applied on the left knees of all the rabbits via medial parapatellar arthrotomy to simulate knee instability. Immediately after the surgical procedure, 0.2 cc of EVOO was injected into the knee joint of rabbits numbered 1-16, and the control group received 0.2 cc of sterile saline. On the 14th day, long-term group subjects were administered another dose of 0.2 cc EVOO intraarticularly.
The gross morphological scores of the control group subjects were significantly different from the EVOO group for both short-term (p = 0,055) and long-term (p = 0,041) scores. In parallel, the MRI results of the EVOO subjects were significantly different from the control group for both short-term and long-term follow-up assessment scores (p = 0.017, p = 0.014, respectively). The Mankin scoring results showed that there were statistically significant differences between the EVOO and control group in the comparison of both total scores (p = 0.001 for short-term and p = 0.004 for long-term) and subgroup scoring, including macroscopic appearance, chondrocyte cell number, staining, and Tidemark integrity in both short-term (p = 0.005, p = 0.028, p = 0.001, p = 0.005, respectively) and long-term assessments (p = 0.002, p = 0.014, p < 0.001, p = 0. 200, respectively).
We have observed promising outcomes of intra-articular application of extra virgin olive oil in the treatment of acute degenerative osteoarthritis in rabbit knees. Due to its potential cartilage restorative and regenerative effects, EVOO, when administered intra-articularly, may be a promising agent to consider for further research in the treatment of OA.
本研究旨在通过观察形态学、组织学和影像学表现，分析关节内特级初榨橄榄油（EVOO）注射治疗兔膝骨关节炎（OA）的效果。
本研究共纳入 32 只新西兰大白兔。将随机编号的研究对象分为两组。第 1 至 16 号兔子被纳入 EVOO 组，其余兔子被纳入对照组。两组动物再分别分为短期（5 周）和长期（10 周）随访亚组。所有兔子均经膝关节内侧髌旁入路行前交叉韧带切断术，以模拟膝关节不稳定。术后即刻，将 0.2 cc 的 EVOO 注入第 1 至 16 号兔子的膝关节内，对照组则注射 0.2 cc 的无菌生理盐水。第 14 天，长期组再次接受 0.2 cc 的 EVOO 关节内注射。
对照组的大体形态学评分在短期（p=0.055）和长期（p=0.041）随访时均显著高于 EVOO 组。同时，EVOO 组的 MRI 结果在短期和长期随访时均显著优于对照组（p=0.017，p=0.014）。Mankin 评分显示，EVOO 组与对照组的总评分（短期：p=0.001；长期：p=0.004）及亚组评分（包括大体形态、软骨细胞数量、染色和软骨下嵴完整性）均存在统计学差异（短期：p=0.005，p=0.028，p=0.001，p=0.005；长期：p=0.002，p=0.014，p<0.001，p=0.200）。
我们观察到关节内应用特级初榨橄榄油治疗兔膝关节急性退行性骨关节炎具有良好的效果。由于其具有潜在的软骨修复和再生作用，EVOO 关节内注射可能是治疗 OA 的一种有前途的药物，值得进一步研究。
