来自大肠杆菌的插入硒代半胱氨酸的乳白抑制子丝氨酸tRNA在结构和修饰方面非常独特。
The selenocysteine-inserting opal suppressor serine tRNA from E. coli is highly unusual in structure and modification.
作者信息
Schön A, Böck A, Ott G, Sprinzl M, Söll D
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
出版信息
Nucleic Acids Res. 1989 Sep 25;17(18):7159-65. doi: 10.1093/nar/17.18.7159.
Abstract
Selenocysteine is cotranslationally incorporated into selenoproteins in a unique pathway involving tRNA mediated suppression of a UGA nonsense codon (1-3). The DNA sequence of the gene for this suppressor tRNA from Escherichia coli predicts unusual features of the gene product (4). We determined the sequence of this serine tRNA (tRNA(UCASer]. It is the longest tRNA (95 nt) known to date with an acceptor stem of 8 base pairs and lacks some of the 'invariant' nucleotides found in other tRNAs. It is the first E. coli tRNA that contains the hypermodified nucleotide i6A, adjacent to the UGA-recognizing anticodon UCA. The implications of the unusual structure and modification of this tRNA on recognition by seryl-tRNA synthetase, by tRNA modifying enzymes, and on codon recognition are discussed.
摘要
硒代半胱氨酸通过一种独特的途径共翻译掺入硒蛋白中，该途径涉及tRNA介导的对UGA无义密码子的抑制(1-3)。来自大肠杆菌的这种抑制性tRNA基因的DNA序列预测了该基因产物的异常特征(4)。我们确定了这种丝氨酸tRNA（tRNA[UCASer]）的序列。它是迄今为止已知最长的tRNA（95个核苷酸），其接受茎有8个碱基对，并且缺少其他tRNA中发现的一些“不变”核苷酸。它是第一个含有与识别UGA的反密码子UCA相邻的超修饰核苷酸i6A的大肠杆菌tRNA。讨论了这种tRNA异常结构和修饰对丝氨酰-tRNA合成酶识别、tRNA修饰酶识别以及密码子识别的影响。
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Nucleic Acids Res. 1989 Sep 25;17(18):7159-65. doi: 10.1093/nar/17.18.7159.
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本文引用的文献
1
N-FORMYL-METHIONYL-S-RNA.N-甲酰甲硫氨酰-S-核糖核酸
J Mol Biol. 1964 Jun;8:835-40. doi: 10.1016/s0022-2836(64)80164-9.
2
The need for selenite and molybdate in the formation of formic dehydrogenase by members of the coli-aerogenes group of bacteria.大肠杆菌-产气杆菌菌群细菌形成甲酸脱氢酶过程中对亚硒酸盐和钼酸盐的需求。
Biochem J. 1954 May;57(1):10-6. doi: 10.1042/bj0570010.
3
Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides.tRNA的定量酶促水解：tRNA核苷的反相高效液相色谱法
J Chromatogr. 1982 Jul 9;230(2):297-308.
4
Resolution of distinct selenium-containing formate dehydrogenases from Escherichia coli.大肠杆菌中不同含硒甲酸脱氢酶的解析
J Bacteriol. 1981 Mar;145(3):1317-24. doi: 10.1128/jb.145.3.1317-1324.1981.
5
Selenium-dependent enzymes.硒依赖性酶。
Annu Rev Biochem. 1980;49:93-110. doi: 10.1146/annurev.bi.49.070180.000521.
6
Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.来自牛肝脏的乳白抑制因子丝氨酸tRNA形成磷酰丝氨酰-tRNA。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6215-9. doi: 10.1073/pnas.79.20.6215.
7
Translational efficiency of transfer RNA's: uses of an extended anticodon.转运RNA的翻译效率：扩展反密码子的用途。
Science. 1982 Nov 12;218(4573):646-52. doi: 10.1126/science.6753149.
8
The effect of point mutations affecting Escherichia coli tryptophan tRNA on anticodon-anticodon interactions and on UGA suppression.影响大肠杆菌色氨酸tRNA的点突变对抗密码子-反密码子相互作用及UGA抑制的作用
J Mol Biol. 1984 Aug 5;177(2):329-42. doi: 10.1016/0022-2836(84)90460-1.
9
Transfer RNA as a cofactor coupling amino acid synthesis with that of protein.转运RNA作为一种辅助因子，将氨基酸合成与蛋白质合成联系起来。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):229-36. doi: 10.1073/pnas.61.1.229.
10
Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.酪氨酸转运核糖核酸中的碱基修饰：一种影响核糖体结合的修饰碱基。
J Mol Biol. 1969 Jan 14;39(1):145-57. doi: 10.1016/0022-2836(69)90339-8.
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